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  • Recombinant Human MMP-3
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP259FNdatasheet pdfdatasheet pdf10 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Human MMP-3 Antigen produced in E. coli represents human MMP-3 (matrix metalloproteinase 3) containing the entire catalytic N-terminal domain and C-terminal domain. MMP-3 is otherwise known as stromelysin-1, a member of a family of secreted zinc-dependent endoproteases, playing a role in the degradation of extracellular matrix (ECM) components, including collagens, laminin, basement membrane glycoprotein and fibronectin.

      MMP-3 is implicated in wound repair, and tumor initiation, and studies have shown high expression of MMP-3 by monocytes, and that MMP-3 is also expressed by microglial cells, chondrocytes, and by fibroblast-like synoviocytes in patients with rheumatoid arthritis (RA) and osteoarthritis.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant MMP-3 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      MMP-3 activity was measured by its ability to cleave a chromogenic peptide MMP-3 substrate at room temperature. At a MMP-3 concentration of 2.5 µg/ml, 50% cleavage was achieved at an incubation time of approximately 75 mins.
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Molecular Weight
      42.8kD (378 amino acid residues) containing the entire catalytic N-terminal domain and C-terminal domain.
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
    • Test Principle
    • Buffer Solution
      10mM Sodium Phosphate
      .1% CHAPS
      0.1mM Calcium Chloride
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      intracellular membrane-bounded organelle
      extracellular space
      metalloendopeptidase activity
      proteinaceous extracellular matrix
      calcium ion binding
      zinc ion binding
      collagen catabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MMP-3 Formats

    Formats Applications Sizes available
    MMP-3 : Rec. Protein FN 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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