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  • Native Human MMP-3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5980-0357Edatasheet pdf5 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Intended Use
    • Target Species
    • Product Form
      Purified Protein form human synovial fibroblasts - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.05%Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      0.33 mg/ml
    • Molecular Weight
      Exhibits a doublet at 59/57 kDa which after activation with APMA reduces to a 50/38 kDa doublet that remains fairly stable.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline, pH 7.5
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      collagen catabolic process
      proteinaceous extracellular matrix
      extracellular space
      calcium ion binding
      metalloendopeptidase activity
      zinc ion binding
      intracellular membrane-bounded organelle
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Enzyme is in the pro-enzyme form and therefore needs activating prior to use by the following method. Activation is achieved by the addition of 10% (v/v) of a 20 mM solution ofaminophenyl mercuric acetate in DMSO, followed by incubation at 37ºC for 8 hours. (If time is short substantial activation is achieved within 3 hours).

    Additional MMP-3 Formats

    Formats Applications Sizes available
    MMP-3 : Purified E 5 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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