This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Instructions For Use
Enzyme is in the pro-enzyme form and therefore needs activating prior to use by the following method. Activation is achieved by the addition of 10% (v/v) of a 20 mM solution ofaminophenyl mercuric acetate in DMSO, followed by incubation at 37ºC for 8 hours. (If time is short substantial activation is achieved within 3 hours).