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MMP-1

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  • Recombinant Human MMP-1

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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP257FNdatasheet pdfdatasheet pdf10 µg
    PHP257
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human MMP-1 antigen produced in E. coli corresponds to human MMP-1 (matrix metalloproteinase 1) containing the entire catalytic N-terminal domain and the C-terminal domain. MMP-1 otherwise known as interstitial collagenase, is a member of a family of secreted zinc-dependent endoproteases, which play a role in the degradation of extracellular matrix (ECM) components, including both fibrillar and non-fibrillar collagens, laminin, basement membrane glycoprotein and fibronectin.

      MMP-1 specifically cleaves types I, II, III, VII and X collagens, and also interacts with and cleaves the secreted viral Tat protein during HIV infection, resulting in a decrease in neuronal Tat's mediated neurotoxicity.

      MMP-1 is overexpressed in invasive melanoma (Ye et al. 2001), colorectal (Hinoda et al. 2001) and oesophageal (Yamashita et al. 2001) cancers, and has been implicated in arthritis, atherosclerotic lesion formation, and in the repair processes of the heart after myocardial infarction (Creemers et al. 2001).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified recombinant MMP-1 expressed in E.coli
    • Preservative Stabilisers
      None present
    • Activity
      MMP-1 activity was measured by its ability to cleave a chromogenic peptide MMP-1 substrate at room temperature. At an MMP-1 concentration of 2.5 μg/ml, 50% cleavage was achieved at an incubation time of approximately 25 mins.
    • Purity
      >90% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <1EU/ug
    • Test Principle
    • Buffer Solution
      Sodium phosphate
      Calcium chloride
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      3 months from date of reconstitution.
    • GO Terms
      GO:0044419
      interspecies interaction between organisms
      Related reagents
      GO:0007596
      blood coagulation
      Related reagents
      GO:0005509
      calcium ion binding
      Related reagents
      GO:0050900
      leukocyte migration
      Related reagents
      GO:0030574
      collagen catabolic process
      Related reagents
      GO:0004222
      metalloendopeptidase activity
      Related reagents
      GO:0005578
      proteinaceous extracellular matrix
      Related reagents
      GO:0008270
      zinc ion binding
      Related reagents
      GO:0006508
      proteolysis
      Related reagents
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MMP-1 Formats

    Formats Applications Sizes available
    MMP-1 : Rec. Protein FN 10 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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