MIF Antibody (PrecisionAbTM Antibody)

MIF Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with MIF antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 150 second exposure. Arrow points to MIF (molecular weight 13 kDa)

  • MIF Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • MIF Antibody with Control Lysate
  • Goat anti MIF
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    MIF antibody recognizes macrophage migration inhibitory factor, also known as MIF, glycosylation-inhibiting factor, GIF, L-dopachrome isomerase, L-dopachrome tautomerase or phenylpyruvate tautomerase. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00057WBdatasheet pdfdatasheet pdf100 µl
      VPA00057KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Goat anti Human MIF antibody recognizes MIF, also known as macrophage migration inhibitory factor. In humans, the MIF protein is encoded by the MIF gene. This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.

        Goat anti Human MIF antibody detects MIF as a single band of 13 kDa in a wide range of human cell line lysates.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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