MEK 6 Antibody (PrecisionAbTM Antibody)

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MEK 6 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with MEK 6 antibody followed by detection with Goat anti Mouse HRP conjugated (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 18 second exposure. Arrow points to MEK 6 (molecular weight 38 kDa).

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  • MEK 6 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti MEK 6
  • MEK 6 Antibody with Control Lysate
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    MEK 6 antibody recognizes the dual specificity mitogen-activated protein kinase kinase 6, also known as MAPK/ERK kinase 6, MAPKK 6, SAPK kinase 3 or stress-activated protein kinase kinase 3.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG1
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00672WBdatasheet pdfdatasheet pdf100 µl
      VMA00672
      VMA00672KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00672KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Mouse anti Human MEK 6 antibody recognizes the dual specificity mitogen-activated protein kinase kinase 6, also known as MAPK/ERK kinase 6, MAPKK 6, SAPK kinase 3 or stress-activated protein kinase kinase 3.

        The MAP2K6 gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, MEK 6 is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis (provided by RefSeq, Jul 2008).

        Mouse anti MEK 6 antibody detects a band of 38 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
        Human
      • Western Blotting
        Mouse anti MEK 6 antibody detects a band of approximately 38 kDa in Jurkat cell lysates
        Mouse anti MEK 6 antibody detects a band of approximately 38 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatograpy from ascites
        20μl Mouse monoclonal antibody purified by affinity chromatograpy from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        E coli-derived recombinant protein of partial human MEK 6
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        muscle cell differentiation
        protein binding
        Toll signaling pathway
        MyD88-dependent toll-like receptor signaling pathway
        MyD88-independent toll-like receptor signaling pathway
        nucleoplasm
        ATP binding
        protein serine/threonine kinase activity
        protein tyrosine kinase activity
        cytosol
        toll-like receptor 2 signaling pathway
        MAP kinase kinase activity
        toll-like receptor 4 signaling pathway
        activation of MAPK activity
        toll-like receptor 3 signaling pathway
        cell cycle arrest
        DNA damage induced protein phosphorylation
        innate immune response
        positive regulation of muscle cell differentiation
        toll-like receptor 1 signaling pathway
        stress-activated MAPK cascade
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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