MEK 1 Antibody (PrecisionAbTM Antibody)

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MEK 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with MEK 1 antibody followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/2500, STAR126P) and visualized on the ChemiDoc MP with 60 second exposure. Arrow points to MEK 1 (molecular weight 42 kDa)

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  • MEK 1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • MEK 1 Antibody with Control Lysate
  • Human anti MEK 1
  • MEK 1 Antibody with Control Lysate
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    MEK 1 antibody recognizes dual specificity mitogen-activated protein kinase kinase 1, also known as MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1 or MEK 1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00010WBdatasheet pdfdatasheet pdf100 µl
      VMA00010
      VMA00010KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00010K
      VMA00010KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00010KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human MEK 1 antibody recognizes human dual specificity mitogen-activated protein kinase kinase 1, also known as MEK 1 or MAPKK1. MEK 1 is a 393 amino acid, dual specificity protein kinase which has the ability to phosphorylate both threonine and tyrosine residues. MEK1 acts downstream of Ras and Raf and is responsible for the phosphorylation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK). It is an integral component of the MAP kinase signalling cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.

        Human anti Human MEK 1 antibody recognizes human MEK1 as a band of ~42 kDa in multiple human cell line lysates by western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti MEK 1 antibody detects a band of approximately 42 kDa in Jurkat cell lysates.
        Anti MEK 1 antibody detects a band of approximately 42 kDa in Jurkat cell lysates.
        Anti MEK 1 antibody detects a band of approximately 42 kDa in Jurkat cell lysates.
      • Product Form
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
      • Preparation
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Full length recombinant human MEK 1 expressed in Sf9 cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        activation of MAPK activity
        toll-like receptor 2 signaling pathway
        cellular senescence
        nerve growth factor receptor signaling pathway
        MyD88-independent toll-like receptor signaling pathway
        Ras protein signal transduction
        cytosol
        MAP kinase kinase activity
        axon guidance
        innate immune response
        Toll signaling pathway
        ATP binding
        negative regulation of cell proliferation
        cell cycle arrest
        protein tyrosine kinase activity
        toll-like receptor 1 signaling pathway
        insulin receptor signaling pathway
        epidermal growth factor receptor signaling pathway
        toll-like receptor 3 signaling pathway
        plasma membrane
        protein serine/threonine kinase activity
        stress-activated MAPK cascade
        toll-like receptor 4 signaling pathway
        activation of MAPKK activity
        MyD88-dependent toll-like receptor signaling pathway
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR126P
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
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        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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