MEK 1 Antibody | AbD05812

MEK 1 Antibody | AbD05812 gallery image 1

HeLa lysate probed with Human anti Human MEK1 (HCA067)

MEK 1 Antibody | AbD05812 gallery image 2

Western blot analysis of MCF-7 human breast adenocarcinoma whole cell lysate probed with Human anti Human MEK-1 antibody (HCA067) followed by HRP conjugated F(ab')2 Goat anti Human IgG, visualized by chemiluminescence

  • MEK 1 Antibody | AbD05812 thumbnail image 1
  • MEK 1 Antibody | AbD05812 thumbnail image 2
  • Human anti Human MEK 1
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA067E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Human anti Human MEK 1 antibody, clone 5812 recognizes human Dual specificity mitogen-activated protein kinase kinase 1, also known, MEK 1 or MAPKK1. MEK 1 is a 393 amino acid ~43 kDa dual specificity protein kinase which has the ability to phosphorylate both threonine and tyrosine residues. MEK1 acts downstream of Ras and Raf and is responsible for the phosphorylation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase (MAPK). It is an integral component of the MAP kinase signalling cascade that regulates cell growth and differentiation, and may also play a role in brain synaptic plasticity.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/250

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      HCA067 detects a band of approximately 43kDa in HCT-116 cell lysates.
    • Instructions For Use

    Additional MEK 1 Antibody Formats

    Formats Clone Applications Sizes available
    MEK 1 Antibody : Purified AbD05812 E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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