MDC1 Antibody

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MDC1 Antibody gallery image 1

Published customer image:
Sheep anti Human MDC1 antibody (AHP799) used for the visualization of MDC1 expression in U2OS osteosarcoma cells following irradiation induced DNA damage by immunofluorescence.
Image caption:
PP4 dephosphorylates γH2AX at the sites of DNA damage. (A,B) U2OS cells transfected with siRNAs against PP4C, PP2ACα/β or a control sequence were irradiated with 1.5 Gy and processed for γH2AX (A,B) and MDC1 (B) immunofluorescence and DAPI counterstaining at the indicated time points. Images were taken with a × 40 objective (A) or with a × 63 objective (B). Scale bar, 23 μm (A) and 16 μm (B). (C–F) Quantification of cells with >5 γH2AX (C), >10 γH2AX (D,F) and >5 MDC1 (E) foci. At least 300 cells were analysed per experiment. Cells were treated with KU55933 and KU57788 12 min post-IR (F). Error bars represent standard error of mean of independent duplicated experiments (C) or standard deviation of three independent experiments (D–F). A × 63 oil immersion objective (C,E) or a × 40 water immersion objective (D,F) was used. (G) U2OS cells transfected with siRNAs against PP4C or a control sequence were collected at the indicated time points immediately before (no IR), or 1 or 6 h after irradiation (10 Gy), then fractionated as described in Méndez & Stillman (2000) into whole-cell lysates (WCLs), nuclear soluble (S3) or chromatin (P3) fractions, and analysed by immunoblotting using the indicated antibodies. DAPI, 4,6-diamidino-2-phenylindole; H3, histone H3; IR, irradiation; MDC1, mediator of DNA damage checkpoint 1; siRNA, small interfering RNA.

From: Nakada S, Chen GI, Gingras AC, Durocher D.
PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint.
EMBO Rep. 2008 Oct;9(10):1019-26.

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MDC1 Antibody gallery image 2

Published customer image:
Sheep anti Human MDC1 antibody (AHP799) used for the demonstration of MDC1 accumulation of MDC1 in murine spermatazoa by immunofluorescence.
Image caption:
Analysis of epigenetic differences in meiotic silencing in oocytes compared to spermatocytes. a XY pachytene spermatocyte stained for SYCP3 (magenta), HORMAD2 (green) and MDC1 (red), showing MDC1 accumulation in the sex chromatin (arrow). b XO pachytene oocyte stained for SYCP3 (magenta), HORMAD2 (green) and MDC1 (red), also showing MDC1 enrichment on the asynapsed X chromosome (arrow). c XY pachytene spermatocyte stained for SYCP3 (magenta), HORMAD2 (green) and SUMO-1 (red), showing accumulation of SUMO-1 in the sex chromatin (arrow). d XO pachytene oocyte stained for SYCP3 (magenta), HORMAD2 (green) and SUMO-1 (red), also showing SUMO-1 enrichment on the asynapsed X chromosome (arrow). e XY diplotene spermatocyte stained with SYCP3 to mark chromosome axes (magenta), HORMAD2 (green) to label the asynapsed X and Y chromosomes (arrow) and H3K9me3 (red), which shows enrichment in the chromatin of the X and Y chromosomes and also at constitutive heterochromatin (asterisks). A line (yellow) was drawn through the asynapsed X (arrow) to quantify HORMAD2 and H3K9me3 intensities (g). f XO diplotene oocyte showing no enrichment of H3K9me3 in the chromatin of the asynapsed X chromosome (arrow). H3K9me3 staining is restricted to sites of constitutive heterochromatin (asterisks). A line (yellow) was drawn through the asynapsed X (arrow) to quantify HORMAD2 and H3K9me3 intensities (h). g, h Intensities of H3K9me3 and HORMAD2 immunostaining quantified by densitometry across the indicated paths along (g) the asynapsed X in the spermatocyte from panel e, and along (h) the asynapsed X in diplotene XO oocyte from panel f. Scale bars?=?10 μm

From: Cloutier JM, Mahadevaiah SK, ElInati E, Tóth A, Turner J.
Mammalian meiotic silencing exhibits sexually dimorphic features.
Chromosoma. 2016 Jun;125(2):215-26.

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  • Sheep anti Human MDC1 (aa1883-2089)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP799IF, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP799
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Human MDC1 antibody recognizes human Mediator of DNA damage checkpoint protein 1, also known as MDC1 or Nuclear factor with BRCT domains 1. MDC1 is a 2089 amino acid, ~250 kDa nuclear protein containing two BRCT domains and a single FHA domain. MDC1 is involved in checkpoint mediated cell cycle arest in response to DNA damage (UniProt: Q14676)
    • Intended Use
    • Target Species
      Human
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human MDC1 (3838) were raised by repeated immunisations of sheep with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Recombinant MDC1 BRCT domains (aa 1883 - 2089).
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/500
      Western Blotting1/2000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP799 detects 3 bands of approximately 250 kDa in HeLa and 293T cell lysates.
    • Instructions For Use

    Additional MDC1 Antibody Formats

    Formats Applications Sizes available
    MDC1 Antibody : Serum IF, WB 0.1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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