Western blotting analysis of A549 lysate (35µg protein in RIPA buffer) probed with Goat anti Human MCT2 antibody (AHP728) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human MCT2 antibody recognizes human monocarboxylate transporter 2, also known as MCT2 or Solute carrier family 16 member 7. MCT2 is a 478 amino acid ~52 kDa multi-pass transmembrane transporter molecule involved in the transport of pyruvate and lactate across cell membranes.
Northern blot analysis reveals expression of a larger transcript in most normal tissues compared to that which predominates in many cell lines (Lin et al. 1998) and this may be reflected in the slightly lower molecular weight band detected in lung carcinoma cell lysate by this antibody in western blots.
Purified IgG - liquid
Antisera to human MCT2 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Bovine Serum Albumin
Synthetic peptide SNAQSVTSERETNI derived from C terminus of human MCT2.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP728 detects bands of approximately 40 kDa and 60-70 kD in Raji cell lysates.