MCM4 Antibody

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Detection of human MCM4 in whole cell lysates of HeLa cells by Western blotting using Goat anti Human MCM4 (AHP840)

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  • Goat anti Human MCM4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP840P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP840
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human MCM4 antibody recognizes DNA replication licensing factor MCM4, also known as human minichromosome maintenance 4 (MCM4) or CDC21 homolog. Human MCM4 is an 863 amino acid, ~95kDa nuclear component of the MCM complex containing a single MCM domain.

      MCM4 is involved in the initiation and control of eukaryotic DNA replication. Studies show that MCM4 forms a hexameric protein complex with MCM6 and 7, which has DNA helicase activity, and may act as a DNA unwinding enzyme (Kanter et al. 2008). The phosphorylation of MCM4 by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex (Ishimi and Komamura-Kohno 2001).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/20001/10000
      Immunohistology - Paraffin(1)1/2001/1000
      (1)
      Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP840 detects a band of approximately 100 kDa in HeLa cell lysates.
    • Instructions For Use

    Additional MCM4 Antibody Formats

    Formats Applications Sizes available
    MCM4 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

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