MCM4 Antibody

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Detection of human MCM4 in whole cell lysates of HeLa cells by Western blotting using Goat anti Human MCM4 (AHP840)

  • MCM4 Antibody thumbnail image 1
  • Goat anti Human MCM4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP840P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human MCM4 antibody recognizes DNA replication licensing factor MCM4, also known as human minichromosome maintenance 4 (MCM4) or CDC21 homolog. Human MCM4 is an 863 amino acid, ~95kDa nuclear component of the MCM complex containing a single MCM domain.

      MCM4 is involved in the initiation and control of eukaryotic DNA replication. Studies show that MCM4 forms a hexameric protein complex with MCM6 and 7, which has DNA helicase activity, and may act as a DNA unwinding enzyme (Kanter et al. 2008). The phosphorylation of MCM4 by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex (Ishimi and Komamura-Kohno 2001).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/20001/10000
      Immunohistology - Paraffin(1)1/2001/1000
      Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. Bio-Rad recommend citrate buffer, pH6.0, for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP840 detects a band of approximately 100 kDa in HeLa cell lysates.
    • Instructions For Use

    Additional MCM4 Antibody Formats

    Formats Applications Sizes available
    MCM4 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting


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