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MAPKAP1 Antibody

MAPKAP1 Antibody gallery image 1

Western blot analysis of human skeletal muscle lysate probed with Rabbit anti Human MAPKAP1 (AHP1711) at 1(A) and 2(B) ug/ml

MAPKAP1 Antibody gallery image 2

Immunohistochemical staining of human skeletal muscle with Rabbit anti Human MAPKAP1 (AHP1711)

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  • MAPKAP1 Antibody thumbnail image 2
  • Rabbit anti MAPKAP1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1711P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti MAPKAP1 antibody recognizes the human Mitogen activated protein kinase associated protein 1 (MAPKAP1).

      MAPKAP1 forms and essential component of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation. It is believed to modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation and also inhibits HRAS and KRAS signaling. MAPKAP1 enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.

      MAPKAP1 is a ubiquitously expressed protein with highest levels being detected in the heart and skeletal muscle. Multiple alternatively spliced isoforms of MAPKAP1 have been identified.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to Human MAPKAP1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      A peptide corresponding to a 18 amino acid sequence from near the centre of Human MAPKAP1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1.02.0ug/ml
      Immunohistology - Paraffin(1)5.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human skeletal muscle
    • Immunofluorescence
    • Western Blotting
      AHP1711 detects bands of approximately 37 and 73kDa in human skeletal muscle lysate
    • Instructions For Use

    Additional MAPKAP1 Antibody Formats

    Formats Applications Sizes available
    MAPKAP1 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Histar Detection SystemSTAR3000A50 TestsC, P
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human skeletal muscle
                • Product Images

                • MAPKAP1 Antibody thumbnail image 1
                • MAPKAP1 Antibody thumbnail image 2

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