MAPK9 / JNK2 Antibody (PrecisionAbTM Antibody)

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MAPK9 / JNK2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with MAPK9 / JNK2 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 115 second exposure. Arrow points to MAPK9 / JNK2 (molecular weight 54 kDa)

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  • MAPK9 / JNK2 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • MAPK9 / JNK2 Antibody with Control Lysate
  • Rabbit anti MAPK9 / JNK2
  • MAPK9 / JNK2 Antibody with Control Lysate
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    Rabbit anti human MAPK9/JNK2 antibody recognizes mitogen-activated protein kinase 9, also known as MAP kinase 9, MAPK 9, Jun kinase, c-Jun N-terminal kinase 2, c-Jun kinase 2, stress-activated protein kinase 1a or stress-activated protein kinase JNK2. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00281WBdatasheet pdfdatasheet pdf100 µl
      VPA00281
      VPA00281KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00281K
      VPA00281KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00281KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human MAPK9 / JNK2 antibody recognizes MAP kinase 9 (MAPK9), also known as c-Jun N-terminal kinase 2, c-Jun kinase 2, stress-activated protein kinase 1a and stress-activated protein kinase JNK2.

        MAPK9 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. MAPK9 and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in non-stressed cells. Studies of MAPK9's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported (provided by RefSeq, Sep 2008).

        Rabbit anti Human MAPK9 / JNK2 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti MAPK9 / JNK2 detects a band of approximately 54 kDa in Jurkat cell lysate
        Anti MAPK9 / JNK2 detects a band of approximately 54 kDa in Jurkat cell lysate
        Anti MAPK9 / JNK2 detects a band of approximately 54 kDa in Jurkat cell lysate
      • Product Form
        Ig fraction - liquid
        Ig fraction - liquid
        Ig fraction - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 349-379 of human MAPK9 / JNK2
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer
        400μg Jurkat lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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