MAPK14 (pThr180/pTyr182) Antibody

MAPK14 (pThr180/pTyr182) Antibody gallery image 1

Western blot analysis of extracts from HeLa cells untreated or treated with TNFa + Calyculin A using Rabbit anti MAPK14 (pThr180/pTyr182) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with TNF-a (20ng/ml) and Calyculin A (100nM) for 30 minutes

  • MAPK14 (pThr180/pTyr182) Antibody thumbnail image 1
  • Rabbit anti MAPK14 (pThr180/pTyr182)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2645WBdatasheet pdfdatasheet pdf50 µl
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti MAPK14 (pThr180/pTyr182) antibody recognizes mitogen-activated protein kinase 14 (MAPK14), also known as cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2 or MAP kinase p38 alpha, when phosphorylated at threonine 180 and tyrosine 182.

      MAPK14 is a serine threonine kinase and a member of the MAP kinase family. MAPK14 signaling plays a key role in the regulation of multiple processes including cell differentiation and proliferation.

      MAPK14 is phosphorylated at threonine 180 and tyrosine 182 by MAP2K3 and MAP2K6. Phosphorylation at threonine 180 and tyrosine 182 activates MAPK14 (Raingeaud et al. 1995).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional MAPK14 (pThr180/pTyr182) Antibody Formats

    Formats Applications Sizes available
    MAPK14 (pThr180/pTyr182) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

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          Recommended Positive Controls

            Histology Controls

              Further Reading

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