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Mammaglobin A Antibody

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  • Goat anti Human Mammaglobin A
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2267E, P *datasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Human mammoglobin A antibody recognizes human mammaglobin A, also known as Secretoglobin family 2A member 2, the product of the SCGB2A2 gene. Mammaglobin A is a ~10.5kDa protein expressed in mammary tissue (Watson et al. 2001).

      Immunohistochemical studies have shown an increase in the sensitivity of breast cancer detection using mammaglobin antibodies, when compared to BRST-1 and GCDFP-15, and that mammaglobin can be utilised to confirm breast origin of metastic tumors in axillary lymph nodes (ALNs), which are used as an important prognostic factor for staging breast cancer, and subsequent treatment (Layque et al. 1999).

      The detection of circulating tumor cells is a useful monitoring tool for the progression of micro-metastases in breast cancer patients with assays based primarily on reverse-transcriptase PCR having been developed (Zach et al. 1999, Ooka et al. 2001).

      The mRNA of human mammaglobin has been found to be the most specific marker for the hematogenous spread of breast cancer cells. Since it is cells from primary breast tumors which are normally present in ALNs, distinguishing between breast epithelial cells and lymphoid cells is beneficial and studies using gene expression analysis have determined that mRNA encoding mammaglobin A protein can distinguish between these cells types.

      Mammaglobin A, a highly specific biomarker for breast tissue, was originally thought to be absent from tumors of non-breast origin. More recent evidence using immunohistochemical studies suggests that the co-expression of mammaglobin A with lipophilin B (SCGB1D2), can be detected in both normal and malignant tissues of the ovary, cervix and uterus (Bignotti et al. 2013).

      Goat anti human mammoglobin A exhibits staining of cytoplasm in some acinar cells of human breast tissue in paraffin sections. This polyclonal antibody is expected to recognize both mammaglobin isoforms listed in UniProt entry Q13296
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to Human mammaglobin A was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-NPQVSKTEYKEL from the internal region of Human mammaglobin A (NP_002402.1)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      steroid binding
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin(1)4.06.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human Breast
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Mammaglobin A Antibody Formats

    Formats Applications Sizes available
    Mammaglobin A Antibody : Purified E, P * 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human Breast

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