Goat anti Human mammoglobin A antibody recognizes human mammaglobin A, also known as Secretoglobin family 2A member 2, the product of the SCGB2A2 gene. Mammaglobin A is a ~10.5kDa protein expressed in mammary tissue (Watson et al. 2001).
Immunohistochemical studies have shown an increase in the sensitivity of breast cancer detection using mammaglobin antibodies, when compared to BRST-1 and GCDFP-15, and that mammaglobin can be utilised to confirm breast origin of metastic tumors in axillary lymph nodes (ALNs), which are used as an important prognostic factor for staging breast cancer, and subsequent treatment (Layque et al. 1999).
The detection of circulating tumor cells is a useful monitoring tool for the progression of micro-metastases in breast cancer patients with assays based primarily on reverse-transcriptase PCR having been developed (Zach et al. 1999, Ooka et al. 2001).
The mRNA of human mammaglobin has been found to be the most specific marker for the hematogenous spread of breast cancer cells. Since it is cells from primary breast tumors which are normally present in ALNs, distinguishing between breast epithelial cells and lymphoid cells is beneficial and studies using gene expression analysis have determined that mRNA encoding mammaglobin A protein can distinguish between these cells types.
Mammaglobin A, a highly specific biomarker for breast tissue, was originally thought to be absent from tumors of non-breast origin. More recent evidence using immunohistochemical studies suggests that the co-expression of mammaglobin A with lipophilin B (SCGB1D2), can be detected in both normal and malignant tissues of the ovary, cervix and uterus (Bignotti et al. 2013).
Goat anti human mammoglobin A exhibits staining of cytoplasm in some acinar cells of human breast tissue in paraffin sections. This polyclonal antibody is expected to recognize both mammaglobin isoforms listed in UniProt entry Q13296
Purified IgG - liquid
Antiserum to Human mammaglobin A was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography using the immunizing peptide
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Synthetic peptide sequence C-NPQVSKTEYKEL from the internal region of Human mammaglobin A (NP_002402.1)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.