Rabbit anti Human MAML1 antibody recognises an epitope within the N-Terminal (NT) region of human MAML1 (Mastermind-like Protein 1), a widely expressed nuclear protein and member of the mastermind family, which acts as a crucial coactivator of Notch-mediated transcriptional activation, and plays a role in hematopoietic development, by acting as a regulator of Notch-mediated lymphoid cell fate decisions.
MAML1 interacts with the Mediator complex component CDK8 (Cyclin-dependent kinase 8), enhancing the phosphorylation and proteolytic turnover of the Notch intracellular domain (NICD) within the nucleus, and forms a DNA-binding complex with RBPSUH/RBP-J kappa/CBF1, playing a vital role in the transcriptional activation of Notch targets. Studies have shown that the NT region of MAML1 interacts with the DNA-binding domain of the tumor suppressor protein p53, and that over-expression of MAML1, greatly increases p53-mediated gene transcription and biological function.
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human MAML1 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Synthetic peptide sequence VLPTCPMAEFALPR from the N-Terminal region of MAML1 (NP_055572.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP2253 detects a band of approximately 110kDa in human skeletal musde cell lysates.