Maf Antibody | 6B8

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Maf Antibody | 6B8 gallery image 1

Western Blot analysis of MAF expression in PC-12 using Mouse anti Human MAF (MCA4938Z)

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  • Mouse anti Human Maf:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    6B8
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4938ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA4938Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Maf antibody, clone 6B8 recognizes human transcription factor Maf, also known as Maf, proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog. Maf is a 403 amino acid ~42 kDa transcriptional regulator.

      Two isoforms of Maf caused by alternative splicing have been described. Mouse anti Human Maf antibody, clone 6B8 recognizes the non-canonical long isoform 1 of Maf, binding to the canonical short isoform of Maf which lacks the C-terminal 30 amino acid sequence of the immunogen used to generate the 6B8 clone has not been evaluated (UniProt : O75444).

      Mutations in the MAF gene can lead to development of Cataract 21, multiple types (CTRCT21) a disease characterized by opacificiation of the eye lens resulting in varying degrees of visual impairment (Jamieson et al. 2002). Mutation in the MAF gene can also lead to the development of Ayme-Gripp syndrome (AYGRP), a multisystem disorder characterized by congenital cataracts, deafness mammary dysplasia and impaired growth (Niceta et al. 2015).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 304 - 404 of human MAF.
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      RNA polymerase II transcription factor activity
      sequence-specific DNA binding
      transcription from RNA polymerase II promoter
      regulation of transcription, DNA-dependent
      chromatin
      nucleus
      sequence-specific DNA binding transcription factor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Maf Antibody Formats

    Formats Clone Applications Sizes available
    Maf Antibody : Preservative Free 6B8 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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