MAdCAM-1 antibody | 17F5
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Mouse anti Human MAdCAM-1
- Product Type
- Monoclonal Antibody
- Clone
- 17F5
- Isotype
- IgG1
- Specificity
- MAdCAM-1
Antibody Quality and Performance Guarantee
| Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1) a ~60 kDa transmembrane protein that belongs to the immunoglobulin superfamily. MAdCAM-1 is strongly expressed on mucosal lymphoid tissue and high endothelial venules of Peyer's patches. Human MAdCAM-1 interacts with the beta 7 integrin LPAM-1 (alpha4beta7), CD62L, and VLA-4 (alpha4beta1) and is involved in lymphocyte trafficking, Mouse anti Human MAdCAM-1 antibody, clone 17F5 recognizes the mucin domain of MAdCAM-1. This antibody does not block the function of MAdCAM-1 (Leung, E. et al. 2004). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Camel - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant soluble MAdCAM-1-fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3X63Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/10 | 1/100 |
| Immunohistology - Paraffin | |
1/50 | 1/100 |
| Western Blotting 1 | |
- 1 Clone 17F5 recognizes human MAdCAM-1 under non-reducing conditions.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Western Blotting
- Mouse anti Human MAdCAM-1 antibody, clone 17F5 detects a band of approximately 60kDa.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for MAdCAM-1 antibody
-
Leung, E. et al. (1996) Cloning of the mucosal addressin MAdCAM-1 from human brain: identification of novel alternatively spliced transcripts.
Immunol Cell Biol. 74 (6): 490-6. -
Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
Immunol Cell Biol. 82 (4): 400-9. -
Al-Mohammed Salem, K.T. et al. (2012) Adhesion Molecules and the Cellular Population of the Normal Camel (Camelus dromedaries) Mammary Glands
The Open Veterinary Science Journal. 6 (1): 15-22. -
Guerra-Pérez N et al. (2015) Retinoic acid imprints a mucosal-like phenotype on dendritic cells with an increased ability to fuel HIV-1 infection.
J Immunol. 194 (5): 2415-23. -
Loiseau, C. et al. (2016) CCR6(-) regulatory T cells blunt the restoration of gut Th17 cells along the CCR6-CCL20 axis in treated HIV-1-infected individuals.
Mucosal Immunol. 9 (5): 1137-50.
- Synonyms
- Mucosal Addressin Cell Adhesion MOLECULE-1
- RRID
- AB_567121
- UniProt
- Q13477
- Entrez Gene
- MADCAM1
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005624 membrane fraction
- GO:0006955 immune response
- GO:0050776 regulation of immune response
MCA2321
148410
167628
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