Macrophages/Histiocytes Antibody | 3A5

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  • Mouse anti Human Macrophages
  • Mouse anti Human Macrophages
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3A5
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1478TC, P *datasheet pdfdatasheet pdf0.2 ml
    MCA1478T
    MCA1478C, P *datasheet pdfdatasheet pdf2 ml
    MCA1478
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human macrophages antibody, clone 3A5 recognizes an antigen expressed by human macrophages, monocytes and histiocytes, but does not stain interdigitating dendritic cells, Langerhans cells, microglial cells, or lymphoid cells. Some staining of basophilic granulocytes and of mast cells may be seen.

      In immunohistochemical studies Mouse anti Human macrophages antibody, clone 3A5 shows partial cross-blocking with some anti-CD68 monoclonal antibodies, suggesting that the epitope may be associated with this antigen.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Tissue Culture Supernatant - liquid
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant serum free
      Tissue Culture Supernatant serum free
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.7%Bovine Serum Albumin
      0.09%Sodium Azide
      0.7%Bovine Serum Albumin
    • Immunogen
      Human spleen cell homogenate, depleted of lymphocytes and erythrocytes
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/101/100
      Immunohistology - Paraffin(1)1/101/100
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/101/100
      Immunohistology - Paraffin(1)1/101/100
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Spleen
    • Histology Positive Control Tissue
      Spleen
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Macrophages/Histiocytes Antibody Formats

    Formats Clone Applications Sizes available
    Macrophages/Histiocytes Antibody : S/N 3A5 C, P * 2 ml | 0.2 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Spleen
              Spleen

              References

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