Macrophage Antibody | PM-2K

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Macrophage Antibody | PM-2K gallery image 1

Published customer image:
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for the demonstration of PM-2K expressing cells by immunocytochemistry.
Image caption:
Expression of markers on macrophages cultured in serum and M-CSF.
PBMC were cultured in SFM at 5×105 cells/ml in 8-well glass slides for 7 days in the presence of 10% FCS and 10 ng/ml M-CSF. Cells were then air-dried, fixed and stained with 5 μg/ml mouse IgG1 monoclonal antibodies against CD13, CD14, CD32, CD163, CD90, mouse IgG1, proyly-4-hydroxylase, 25F9, S100A8/A9, PM-2K, TE-7, and collagen-I. Cells were then counterstained with hematoxylin to identify nuclei. Inserts indicate staining at higher magnification. Bar is 50 μm.

From: Pilling D, Fan T, Huang D, Kaul B, Gomer RH (2009)
Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts.
PLoS ONE 4(10): e7475.

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Macrophage Antibody | PM-2K gallery image 2

Published customer image:
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for the demonstration of tissue macrophages in lung sections by immunofluorescence.
Image caption:
Identification of fibrocytes in pathological samples.
“HOPE-fixed” human lung tissue sections were stained with antibodies against CD32, CD45RO, CD68, CD164, collagen-I, PM-2K, and TE-7. Sections were stained for A) CD45RO (green), CD68 (red) and DAPI (blue). An example of a CD45RO positive lymphocyte is indicated with a solid arrow. Dual labeled CD45RO and CD68 macrophages and fibrocytes are indicated by open arrows. B) CD45RO (green), PM-2K (red) and DAPI (blue). An example of a dual labeled CD45RO and PM-2K tissue macrophage is indicated with a solid arrow. C) CD45RO (green), collagen-I (red), and DAPI (blue). Dual labeled CD45RO and collagen-I positive cells are yellow. D) PM-2K (green), collagen-I (red), and DAPI (blue). A cluster of PM-2K positive alveolar macrophages is indicated with an open arrow. E) CD45RO (green), CD164 (red), and DAPI (blue). An area of CD164 positive fibroblasts is indicated with a solid arrow. A dual labeled CD45RO and CD164 positive fibrocyte is indicated by an open arrow. F) CD45RO (green), collagen-I (red), and TE-7 (blue). Dual positive TE-7/collagen-I fibroblasts are magenta. G) TE-7 (green), CD32 (red), and DAPI (blue). An example of CD32 positive fibrocytes is indicated with an open arrow. H) Nuclei (white), PM-2K (green), collagen (red), and CD32 (blue). A cluster of PM-2K positive alveolar macrophages are indicated with an asterisk. An area of CD32 and collagen positive/PM-2K negative fibrocytes is indicated with an open arrow. An area of collagen positive/CD32 negative fibroblasts is indicated with a solid arrow. Cells were counterstained with DAPI to identify nuclei. Bars are 100 μm.

From: Pilling D, Fan T, Huang D, Kaul B, Gomer RH (2009)
Identification of Markers that Distinguish Monocyte-Derived Fibrocytes from Monocytes, Macrophages, and Fibroblasts.
PLoS ONE 4(10): e7475.

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Macrophage Antibody | PM-2K gallery image 3

Published customer image:
Mouse anti Human macrophage antibody, clone PM-2K (5870-8008) used for identifying tissue macrpohages in macaque tissue sections by immunofluorescence.
Image caption:
DC-SIGN+ cells are macrophages in the colo-rectal mucosa during the first sixteen hours of infection. DC-SIGN+ cells are observed in lymphoid aggregates of R-H4.2 (sacrificed four hours pi; A) and lamina propria of R-H4.1 (sacrificed four hours pi; C) and R-H16.3 (sacrificed sixteen hours pi; B and D). DC-SIGN+ cells express only occasionally MHC-II molecules (A and B, arrows), but co-express PM-2K (C) and CD68 (D) marking them as macrophages. Frozen sections of colo-rectal mucosa were labeled for DC-SIGN (Alexa-488), CD3 (Alexa-350, A, B and D), MHC-II Mamula-DR (Alexa-546, A, B and C) or PM-2K (Alexa-350, C), CD68 (Alexa-546, D) and nuclei were stained with TO-PRO®-3 (A–D). The computer-generated merged images are shown. CR, crypt; LP, lamina propria; S, submucosal connective tissue; TA, T cell area of mucosal lymphoid aggregate; HEV, high endothelial venule.

From: Ribeiro dos Santos P, Rancez M, Prétet J-L, Michel-Salzat A, Messent V, Bogdanova A, et al. (2011)
Rapid Dissemination of SIV Follows Multisite Entry after Rectal Inoculation.
PLoS ONE 6(5): e19493.

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  • Mouse anti Human Macrophage (Tissue and Proliferative Disorders)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    PM-2K
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    5870-8008C*, WBdatasheet pdfdatasheet pdf0.1 mg
    5870-8008
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human macrophages, clone PM-2K, recognizes most tissue macrophages in thymus, spleen, lymph nodes and tonsil. It is positive on Kupffer cells (liver), alveolar macrophages and macrophages in the interstitial tissue of the kidney, pancreas and other organs.

      This antibody does not react with Macrophage Scavenger Receptors AI and AII.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Dogyes
      Hamsterno
      Guinea Pigno
      Pigyes
      Monkeyyes
      Horseno
      Catyes
      Ratno
      Bovineyes
      Goatno
      Rabbitno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.05% Kathon™
      0.5% Bovine Serum Albumin
    • Immunogen
      Cultured human peritoneal macrophages.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml after reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Western Blotting
      Immunohistology - Frozen(1)1/2001/800
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Tonsil
    • Immunofluorescence
    • Western Blotting
      5870-8008 recognizes an antigen of 150 kDa on macrophage cell membranes.
    • Instructions For Use

    Additional Macrophage Antibody Formats

    Formats Clone Applications Sizes available
    Macrophage Antibody : Purified PM-2K C*, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Tonsil
              • Application NameReference Images
                Immunohistology - Paraffin

              Source Reference

              References

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