Recombinant human macrophage colony stimulating factor (M-CSF) expressed in E. coli.
Purified recombinant protein - lyophilised
Reconstitute in sterile distilled water to 0.1-0.5 mg/ml.
The ED50 was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.
>98% by SDS PAGE and HPLC analyses
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
<0.1 ng/µg (1 EU/µg).
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
2. MacDonald, B.R. et al. (1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow cultures J Bone Miner Res. 1: 227 - 233.