LYVE-1 Antibody | 4G1

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LYVE-1 Antibody | 4G1 gallery image 1

Western blot analysis of LYVE 1 expression in the A549 lung carcinoma cell line using Mouse anti Human LYVE-1, clone 4G1 (MCA4629GA)

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  • Mouse anti Human LYVE-1
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  • Product Type
    Monoclonal Antibody
  • Clone
    4G1
  • Isotype
    IgG2b
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4629GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA4629GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti human LYVE-1, clone 4G1 recogniszs human LYVE-1, otherwise known as hyaluronic acid receptor, a type I integral membrane glycoprotein and member of the Link protein superfamily, expressed on the cell surface of both normal and neoplastic lymphatic endothelial cells, which acts a major receptor for the glycosaminoglycan hyaluronan (HA) in both its soluble and immobilized forms.

      The binding of LYVE-1 to HA on lymph vessel walls and not to the endothelium of blood vessels, makes LYVE-1 a powerful marker for the identification of lymphatic endothelial cells, and for distinguishing between lymphatic vessels and blood vasculature (Koukourakis et al. 2005).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Recombinant human LYVE-1 (amino acids 25-235) purified from Escherichia coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      response to wounding
      anatomical structure morphogenesis
      membrane fraction
      transport
      cell-matrix adhesion
      integral to plasma membrane
      cellular component movement
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/5001/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4629GA detects a band of approximately 38 kDa in human A549 (lung carcinoma) cell lysates.
    • Instructions For Use

    Additional LYVE-1 Antibody Formats

    Formats Clone Applications Sizes available
    LYVE-1 Antibody : Purified 4G1 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              Further Reading

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