LYPLA3 Antibody | 3B11

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LYPLA3 Antibody | 3B11 gallery image 1

Western blot analysis of LYPLA3 expression in HeLa using Mouse anti Human LYPLA3:Azide Free (MCA3783Z)

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  • Mouse anti Human LYPLA3:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3B11
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3783ZWBdatasheet pdfdatasheet pdf0.1 mg
    MCA3783Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human LYPLA3 antibody, clone 3B11 recognizes human Group XV phospholipase A2, 1-O-acylceramide synthase, ACS, LCAT-like lysophospholipase, LLPL, Lysophospholipase 3 or Lysosomal phospholipase A2. LYPLA3 is a 379 amino acid ~42 kDa protein lipase.

      Two potential isoforms generated by alternative splicing have been described, both of which contain the immunogen sequence used to generate Mouse anti Human LYPLA3 antibody, clone 3B11, which is therefore expected to recognize both isoforms of LYPLA3.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant protein corresponding to aa 314-413 of human LYPLA3
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells from the Sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular region
      phosphatidylcholine-sterol O-acyltransferase activity
      lysosome
      fatty acid catabolic process
      lysophospholipase activity
      phospholipid binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.110 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional LYPLA3 Antibody Formats

    Formats Clone Applications Sizes available
    LYPLA3 Antibody : Preservative Free 3B11 WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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