LRRK2 Antibody (PrecisionAbTM Antibody)

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LRRK2 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with LRRK2 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 22 second exposure. Arrow points to LRRK2 (molecular weight 286 kDa)

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  • LRRK2 Antibody with Control Lysate
  • LRRK2 Antibody with Control Lysate
  • Goat anti LRRK2
(Rated 0.0 out of 5 based on 0 customer reviews)
    LRRK2 antibody recognizes leucine-rich repeat serine/threonine-protein kinase 2, also known as dardarin. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00118WBdatasheet pdfdatasheet pdf100 µl
      VPA00118
      VPA00118KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00118K
      VPA00118KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00118KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human LRRK2 antibody recognizes leucine-rich repeat serine/threonine-protein kinase 2 (LRRK2), also known as dardarin.

        LRRK2 is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in LRRK2 have been associated with Parkinson disease-8 (provided by RefSeq, Jul 2008).

        Goat anti Human LRRK2 antibody detects a band of 286 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti LRRK2 detects a band of approximately 286 kDa in HeLa cell lysates.
        Anti LRRK2 detects a band of approximately 286 kDa in HeLa cell lysates.
        Anti LRRK2 detects a band of approximately 286 kDa in HeLa cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Goat polyclonal antibody purified by affinity chromatography.
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
        0.02% Sodium Azide (NaN3) 0.5 % BSA
      • Immunogen
        Peptide with the sequence CELAEKMRRTSV, from the internal region of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        positive regulation of programmed cell death
        ATP binding
        GTPase activator activity
        neuromuscular junction development
        GTP binding
        activation of MAPKK activity
        determination of adult lifespan
        peptidyl-serine phosphorylation
        small GTPase mediated signal transduction
        neuron death
        external side of mitochondrial outer membrane
        positive regulation of protein ubiquitination
        protein autophosphorylation
        response to oxidative stress
        MAP kinase kinase activity
        GTP-dependent protein kinase activity
        regulation of locomotion
        protein homodimerization activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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