LOX-1 Antibody

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LOX-1 Antibody gallery image 1

Staining human umbilical vein endothelial cells with Sheep anti Human LOX-1 (AHP952)

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LOX-1 Antibody gallery image 2

Immunoperoxidase staining of cryosection of human tonsil using Sheep anti Human LOX-1 antibody (AHP952) followed by Donkey anti Sheep IgG conjugated to HRP (STAR88P). Detection using DAB

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LOX-1 Antibody gallery image 3

Immunoperoxidase staining of cryosection of human tonsil using Sheep anti Human LOX-1 antibody (AHP952) followed by Donkey anti Sheep IgG conjugated to HRP (STAR88P). Detection using DAB. High power

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LOX-1 Antibody gallery image 4

Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody (AHP952) in red and with Mouse anti Human CD21 antibody (MCA1195) in red. Nuclei are stained blue using DAPI. Merged image on right

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  • Sheep anti Human LOX-1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP952C, F, IP, WBdatasheet pdfdatasheet pdf50 µg
    AHP952
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti LOX-1 antibody recognizes the type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1), a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.

      AHP952 will detect LOX-1 on transfected cells and in recombinant protein reconstitution assays. LOX-1 levels are low in normal resting human tissues so may fall below detectable levels.

      Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.

      Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (Chen, M. et al.)(Sawamura, T. et al.) and inflammatory diseases such as atherosclerosis (Murphy, J.E. et al.) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs) (Li, D. et al. ).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human LOX-1 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Recombinant human extracellular domain LOX-1 protein from bacteria.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      proteolysis
      blood coagulation
      membrane fraction
      integral to plasma membrane
      extracellular region
      blood circulation
      leukocyte migration
      inflammatory response
      sugar binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      Immunohistology - Frozen
      ImmunoprecipitationNeat
      Western Blotting1/101/100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
      For the best results Bio-Rad recommend HISTAR detection kits..
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Sheep anti LOX-1 antibody detects intact 50kDa LOX-1 and also a prominent 32kDa proteolytic fragment in western blotting.
    • Instructions For Use

    Additional LOX-1 Antibody Formats

    Formats Applications Sizes available
    LOX-1 Antibody : Purified C, F, IP, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence

              References

              Further Reading

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