DiI labelled human low densitiy lipoprotein has been used for monitoring Ac-LDL uptake by monocytes/ macrophages in conjunction with excess unlabelled Ac-LDL 5685-3404 (Rahman et al. 2008).
Sample lots of DiI labelled human low densitiy lipoprotein are individually evaluated for the labelling of human skin fibroblasts or P-388D cells grown in lipoprotein deficient medium for 48 hours.
Purified from human serum - liquid
Purified LDL is labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate). The resultant product is then dialysed against 0.15 M NaCl, 0.05 M TRIS (pH 7.4) and 0.3 mM EDTA, sterilised by filtration and aseptically packaged.
Approx. Protein Concentrations
Total protein concentration 0.2 mg/ml
Reagents In The Kit
Preparing The Antibody
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Avoid repeated freezing and thawing.
Please see label for expiry date.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
In vitro Assay
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
1. Barak, L.S. & Webb, W.W. (1981) Fluorescent low density lipoprotein for observation of dynamics of individual receptor complexes on cultured human fibroblasts. J. Cell Biol. 90: 595-604.
2. Pitas, R.E. et al. (1983) Foam cells in explants of atherosclerotic rabbit aortas have receptors for beta-very low density lipoproteins and modified low density lipoproteins.Arteriosclerosis. 3: 2-12.
3. Barak, L.S. & Webb, W.W. (1982) Diffusion of low density lipoprotein-receptor complex on human fibroblasts. J. Cell Biol. 95: 846-52.