Low Density Lipoprotein

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  • Native Human Low Density Lipoprotein (Acetylated):Dil
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2550-0004F, FN, IF*datasheet pdfdatasheet pdf0.2 mg
    2550-0004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Many lots have been evaluated for the specific labelling of bovine aortic endothelium, HUVECS, murine macrophages and the current lot was evaluated on P-388D cells.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified acetylated LDL from human serum, Dil labelled - liquid
    • Reconstitution
    • Preparation
      Purified LDL is acetylated and then labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate). The resultant product is exhaustively dialyzed against 0.15 M NaCl, 0.05 M Tris, pH 7.4, 0.3 mM EDTA, sterilized by filtration and then aseptically packaged.
    • Preservative Stabilisers
      None present.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      This product is stable when handled aseptically and stored at 4ºC. Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Functional Assays
      Immunofluorescence(1)
      (1)
      3% formaldehyde in PBS is required for fixation. Methanol or Acetone can not be used as 2550-0004 is soluble in organic solvents.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Low Density Lipoprotein Formats

    Formats Applications Sizes available
    Low Density Lipoprotein : Purified F, FN, IF* 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry
                Immunofluorescence

              References

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