Lnk Antibody

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Lnk Antibody gallery image 1

Western blotting analysis of MOLT4 lysate probed with Goat anti Human LNK (AHP1003)

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Lnk Antibody gallery image 2

Western blotting analysis of HEK293 cells transiently transfected with human Lnk probed with Goat anti Human Lnk
Lane A: Mock transfected cells. Lane B: transfected cells

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  • Goat anti Human Lnk (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1003WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1003
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human LNK antibodyrecognizes human SH2B adapter protein 3, also known as Lymphocyte-specific adapter protein Lnk or Lymphocyte adapter protein. LNK is a 575 amino acid SH2B adapteer protein containing a single PH domain and a single SH2 domain. Goat anti Human LNK antibody binds to an epitope within the N-terminal region of LNK, which regulates cytokine receptor-mediated and growth factor pathways, and plays an essential role in B cell lymphopoiesis.

      LNK mRNA is preferentially expressed in lymph node and spleen lymphocytes and the expression of LNK in vascular endothelial cells (ECs), increases in response to TNFα. LNK acts as a negative regulator of the pro-inflammatory molecules VCAM-1 and E-selectin induced by TNFα, modulation of P13-kinase and MAPK ERK1/2 activity. LNK is also involved in the negative regulation of Thrombopoietin-mediated cell proliferation and endomitosis in hematopoietic cell lines.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Antisera to LNK (NT) were raised by repeated immunisation of Goat with highly purified antigen.
      Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence NGPALQPSSPC from LNK amino-terminus.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.31.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1003 detects a band of approximately 70kDa in human MOLT4 cell lysates. (Predicted Mwt. 63.2kDa). A band of 68kDA was resolved in HUVECs, See Fitau et al. for details.
      Suitable loading controls:
      Human anti Human actin β, HCA147
      Rat anti tubulin α, MCA77
    • Instructions For Use

    Additional Lnk Antibody Formats

    Formats Applications Sizes available
    Lnk Antibody : Purified WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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