Western blotting analysis of Daudi (A), Jurkat (B) and K562 (C) lysates (35μg protein in RIPA buffer) probed with Goat anti Human livin antibody (AHP648) at 0.5μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human LIVIN (C terminal) antibody recognizes human Baculoviral IAP repeat-containing protein 7, also known as Kidney inhibitor of apoptosis protein, Livin, RING finger protein 50 or Melanoma inhibitor of apoptosis protein, encoded by the BIRC7 gene. Livin is a 298 amino acid, ~30kDa member of the IAP family containing a single BIR repeat and a single RING -type Zinc finger domain. Goat anti human Livin antibody, recognizes an epitope within the C-terminal (CT) region of human Livin. Two isoforms of livin are generated by alternative splicing α or isoform 2, and β or isoform 1, the latter carrying a 18 amino acid deletion toward the C-terminal region of the molecule. However, the deletion seen in Livin-β is outside the sequence used as immunogen for this Goat anti human Livin antibody which is expected to recognize both isoforms of the molecule.
Livin is upregulated in a number of cancer cell types demonstrating both nuclear and cytoplasmic localization in renal cancer cells whilst equivalent non-cancerous kidneys demonstrate only cytoplasmic staining in restricted cell types ( Wagener et al. 2007).
Purified IgG - liquid
Antisera to human LIVIN (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Bovine Serum Albumin
Peptide sequence C-RAPVRSRVRT corresponding to the C-terminal region of LIVIN (UniProt: Q96CA5).
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP648 detects bands of approximately 30 & 35kDa in MOLT4 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.