Livin Antibody

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Western blot analysis of MOLT 4 lysate probed with Goat anti Human LIVIN (AHP648)

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  • Goat anti Human Livin (C-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP648E, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP648
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Human LIVIN (C terminal) antibody recognizes human Baculoviral IAP repeat-containing protein 7, also known as Kidney inhibitor of apoptosis protein, Livin, RING finger protein 50 or Melanoma inhibitor of apoptosis protein, encoded by the BIRC7 gene. Livin is a 298 amino acid, ~30kDa member of the IAP family containing a single BIR repeat and a single RING -type Zinc finger domain. Goat anti human Livin antibody, recognizes an epitope within the C-terminal (CT) region of human Livin. Two isoforms of livin are generated by alternative splicing α or isoform 2, and β or isoform 1, the latter carrying a 18 amino acid deletion toward the C-terminal region of the molecule. However, the deletion seen in Livin-β is outside the sequence used as immunogen for this Goat anti human Livin antibody which is expected to recognize both isoforms of the molecule.

      Livin is upregulated in a number of cancer cell types demonstrating both nuclear and cytoplasmic localization in renal cancer cells whilst equivalent non-cancerous kidneys demonstrate only cytoplasmic staining in restricted cell types ( Wagener et al. 2007).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human LIVIN (CT) were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
      0.5%Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-RAPVRSRVRT corresponding to the C-terminal region of LIVIN (UniProt: Q96CA5).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      DNA fragmentation involved in apoptotic nuclear change
      activation of JUN kinase activity
      anti-apoptosis
      enzyme binding
      cytoplasm
      zinc ion binding
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/4,000
      Western Blotting0.1ug/ml1.0ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP648 detects bands of approximately 30 & 35kDa in MOLT4 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
    • Instructions For Use

    Additional Livin Antibody Formats

    Formats Applications Sizes available
    Livin Antibody : Purified E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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