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Lipocalin 2 Antibody

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Human kidney cell lysate probed with Goat anti Human lipocalin 2 (AHP2137)

Lipocalin 2 Antibody gallery image 2

Paraffin embedded human spleen probed with Goat anti Human lipocalin 2 (AHP2137)

  • Lipocalin 2 Antibody thumbnail image 1
  • Lipocalin 2 Antibody thumbnail image 2
  • Goat anti Human Lipocalin 2
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2137E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • AHP2137 specifically recognises human Lipocalin-2 (LCN2), otherwise known as Neutrophil gelatinase-associated lipocalin (NGAL), a secreted glycoprotein and member of the Lipocalin family, which is highly expressed in the bone marrow, lungs, stomach, liver and adipose tissue, and is implicated in innate immunity and apoptosis.

      LCN2 acts as a protein transporter, through the binding of small lipophilic substances in the hydrophobic core. Expression of LCN2 is up-regulated during inflammation, and plays an important role in the innate immune response, by binding to bacterial iron-containing siderophores, limiting bacterial growth.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human LCN2 was raised by repeated immunisation of with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide sequence C-AILREDKDPQKMY from the internal region of LCN2 (NP_005555.2).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.010.03ug/ml
      Immunohistology - Paraffin(1)2.03.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human spleen
    • Immunofluorescence
    • Western Blotting
      AHP2137 detects a band of approximately 22kDa in kidney cell lysates.
    • Instructions For Use

    Additional Lipocalin 2 Antibody Formats

    Formats Applications Sizes available
    Lipocalin 2 Antibody : Purified E, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human spleen
                • Product Images

                • Lipocalin 2 Antibody thumbnail image 1
                • Lipocalin 2 Antibody thumbnail image 2

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