Lineage Cocktail | UCHT1/TUK4/DJ130c/LT19/2H7/123C3

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  • Human Lineage Cocktail:Alexa Fluor® 700
  • Human Lineage Cocktail:Alexa Fluor® 700
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody Panel
  • Clone
    UCHT1/TUK4/DJ130c/LT19/2H7/123C3
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2692A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2692A700
    MCA2692A700TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2692A700T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human Lineage Cocktail:Alexa Fluor® 700 is a cocktail of antibodies recognising all leucocytes in human peripheral blood, with the exception of dendritic cells and basophils. Dendritic cells in peripheral blood are characterised by the phenotype HLA-DR+ve, Lineage Cocktail -ve. Basophils are HLA-DR -ve. Human Lineage Cocktail:Alexa Fluor® 700 may therefore be used in combination with HLA-DR specific antibodies (e.g. MCA1879PE ) to identify peripheral blood dendritic cells.

      The individual antibodies in this cocktail are specific for cell subsets as follows:-
      CD3 T lymphocytes
      CD14 Monocytes
      CD16 NK cells, granulocytes
      CD19 B lymphocytes
      CD20 B lymphocytes
      CD56 NK cells
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG conjugated to Alexa Fluor®700 - liquid
      Purified IgG conjugated to Alexa Fluor®700 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      Pack Size: 100 Tests/1ml0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      Pack Size: 25 Tests/0.25ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
      Pack Size: 100 Tests/1ml0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      Pack Size: 25 Tests/0.25ml
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      IgG1/IgG2a/IgG1/IgG1/IgG2b/IgG1
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Lineage Cocktail Formats

    Formats Clone Applications Sizes available
    Lineage Cocktail : Alexa Fluor® 700 UCHT1/TUK4/DJ130c/LT19/2H7/123C3 F 25 Tests/0.25ml | 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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