Leptin
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Recombinant Human Leptin
- Product Type
- Recombinant Protein
- Specificity
- Leptin
Antibody Quality and Performance Guarantee
| Recombinant human leptin is a leptin preparation made in E. coli. Leptin is a major adipose-derived hormone and member of the leptin family, which plays a key role in appetite control and the regulation of energy expenditure. Secreted leptin associates with proteins within the bloodstream and is transported to the brain, where it inhibits or up-regulates the activity of neuropeptides such as neuropeptide Y (NPY) and alpha-melanocyte-stimulating hormone respectively. Leptin is a recognised marker of obesity, with a correlation between circulating levels of leptin and body fat mass, and defects in leptin production result in severe obesity. Regulators of leptin expression include insulin, cortisol, cAMP and thiazolidinediones. |
- Target Species
- Human
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended storage, the addition of 0.1% bovine serum abumin (BSA) is recommended.
- Preparation
- Purified recombinant leptin expressed in E.coli. Sterile filtered through a 0.2 micron filter
- Source
- E.coli
- Buffer Solution
- Lyophilized with 0.1% TFA
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Activity
- PHP198 is biologically active in the ob/ob mouse obesity model. The ob/ob mice were treated via intraperitoneal injection once daily at a dose of 5 µg Leptin/gm of body weight for 7 days. Significant effects on body weight, food consumption, and plasma glucose levels were observed to saline-treated controls.
- Purity
- >98% by SDS PAGE and HPLC
- Approx. Protein Concentrations
- 1.0 mg/ml after reconstitution
- Protein Molecular Weight
- 16.0 kDa (147 amino acid residues)
- Endotoxin Level
- < 1.0 EU/ug
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.2 | 0.4ng/well |
| Functional Assays | |
||
| Western Blotting | |
1.5 | 3.0ng/lane |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Recombinant human leptin may be use as a standard for ELISA applications with purified human leptin antibody (AHP1616).
- Western Blotting
- Recombinant human leptin may be used as the positive control for Western Blotting applications with a purified human leptin antibody (AHP1616).
- UniProt
- P41159
- Entrez Gene
- LEP
- GO Terms
- GO:0005615 extracellular space
- GO:0006112 energy reserve metabolic process
- GO:0008343 adult feeding behavior
- GO:0032099 negative regulation of appetite
View more products with LEPTIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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