Western blotting analysis of Mouse (A) and Rat (B) brain lysates (35μg protein in RIPA buffer) probed with Goat anti Human leptin receptor antibody (AHP1396) at 0.1μgml-1 . Primary incubation was 1 hour. Signal was detected by chemiluminescence.
Western blotting analysis of human cerebral cortex lysate (35μg protein in RIPA buffer) probed with Goat anti Human leptin receptor antibody (AHP1396) at 1.0μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence
Goat anti Human Leptin receptor antibody recognizes the human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor involved in the regulation of adipose-tissue mass/body weight through hypothalamic effects.
The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.
Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG - liquid
Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.02% Sodium Azide (NaN3) 0.5% Bovine Serum Albumin
Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1; NP_001003680.1).
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
AHP1396 detects a band of approximately 150kDa in human brain cell lysates.