Lck (pTyr416)/FYN (pTyr416) Antibody

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Lck (pTyr416)/FYN (pTyr416) Antibody gallery image 1

Western blot analysis of extracts from Jurkat cells untreated, treated with UV or treated with H2O2 using Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody.
H2O2 Treatment: Cells were cultured in serum free media for 16-20 hours before treatment with H2O2 (2mM) for 15 minutes
UV Treatment: Cells were treated with UV for 15-30 minutes

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  • Lck (pTyr416)/FYN (pTyr416) Antibody thumbnail image 1
  • Rabbit anti Lck (pTyr416)/FYN (pTyr416)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2641WBdatasheet pdfdatasheet pdf50 µl
    AHP2641
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti LCK (pTyr416)/FYN (pTyr416) antibody recognizes LCK also known as lymphocyte cell-specific protein-tyrosine kinase, when phosphorylated at tyrosine 416 and FYN also known as Src-like kinase, when phosphorylated at tyrosine 416 FYN.

      LCK and FYN are members of the Src family of tyrosine kinases that are involved in the regulation of numerous processes including differentiation and proliferation. Autophosphorylation of LCK and FYN at tyrosine 416 increases kinase activity via an open confirmation of the LCK and FYN which makes substrates more accessible (Boggon and Eck 2004, Irtegun et al. 2004).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Due to the presence of phosphoproteins in milk, the use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional Lck (pTyr416)/FYN (pTyr416) Antibody Formats

    Formats Applications Sizes available
    Lck (pTyr416)/FYN (pTyr416) Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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        Useful Reagents

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          Recommended Positive Controls

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              Further Reading

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