Lck (pTyr394) antibody
Rabbit anti Lck (pTyr394)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Lck (pTyr394)
Antibody Quality and Performance Guarantee
| Rabbit anti LCK (pTyr394) antibody recognizes LCK, also known as tyrosine-protein kinase Lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific protein-tyrosine kinase or p56-LCK, when phosphorylated at tyrosine 394. LCK is a member of the Src family of tyrosine kinases. The T cell antigen receptor (TCR) depends on LCK to initiate signaling. LCK is autophosphorylated at tyrosine 394 in response to TCR engagement (Wright et al. 1994). Phosphorylation of LCK at tyrosine 394 leads to phosphorylation of CD3 and the gamma chains of the TCR as part of T cell activation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to LCK (pTyr394) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.05% ProClin 300
50% Glycerol - Immunogen
- Phospho specific-peptide corresponding to residues surrounding tyrosine 394 of human LCK
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting |  |
1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Wright, D.D. et al. (1994) Oncogenic activation of the Lck protein accompanies translocation of the LCK gene in the human HSB2 T-cell leukemia.
Mol Cell Biol. 14 (4): 2429-37.
AHP2640
141597
164381
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