Lck Antibody | LCK-01

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  • Mouse anti Human Lck
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LCK-01
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2380C, F *, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2380
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Lck antibody, clone Lck-01 recognizes Tyrosine-protein kinase Lck, also known as Lck, p56lck, Leukocyte C-terminal Src kinase, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck or T cell-specific protein-tyrosine kinase. Lck is a 508 amino acid ~56 kDa cytosolic non-receptor protein tyrosine kinase, primarily expressed in T lymphocytes and natural killer cells.

      Lck is a pivotal enzyme for normal T cell development and TCR-mediated signal transduction. Lck is physically associated with the cytoplasmic region of T cell specific molecules CD4 and CD8 (Bolen & Veillette 1989) and plays an important role in the phosphorylation of several proteins further downstream in the T cell signalling cascade (Veillette et al. 1988).

      Mutations of the Lck gene can lead to immunodeficiency 22 (IMD22), a condition characterized by T-cell dysfunction, patients presenting with recurrent infections, diarrhea and general malaise (Goldman et al. 1998).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Synthetic peptide corresponding to amino acids 22-36 of human lck.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Flow Cytometry(1)
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Clone LCK-01 detects a band of approximately 56kDa in Jurkat cell lysates.
    • Instructions For Use

    Additional Lck Antibody Formats

    Formats Clone Applications Sizes available
    Lck Antibody : Purified LCK-01 C, F *, IP, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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