Laminin Antibody

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Western blot analysis of cell and tissue lysates using Rabbit anti laminin antibody

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Published customer image:
Rabbit anti Human laminin antibody (AHP2491) used for the visualization of laminin in normal colon and in colonic adenocarcinoma tissue sections by immunofluorescence.
Image caption:
Representative indirect immunofluorescence images showing double-stained sections for the detection of the α1 integrin subunit and laminin in a normal colon mucosa (A and B) and a moderately differentiated colon adenocarcinoma specimen (C, D). The α1 subunit (red signal) was observed at the basolateral domain of normal epithelial cells of the lower crypt compartment (LC in panel A and E in panel B) compared to weak signal in the upper compartment (UC, panel A). In CRC, α1 was also localized at the basolateral domain of tumour epithelial cells (E) as well as in the adjacent subepithelial myofibroblasts (MF) (Panels C, D). Basement membrane (arrows) located at the interface between the two tissues was stained with an anti-laminin antibody (green staining). Nuclei were stained with DAPI (blue). Scale bar?=?50 μm.

From: Boudjadi S, Carrier JC, Beaulieu JF.
Integrin α1 subunit is up-regulated in colorectal cancer.
Biomark Res. 2013 Mar 7;1(1):16.

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  • Rabbit anti Laminin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2491WBdatasheet pdfdatasheet pdf50 µl
    AHP2491
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti laminin antibody recognizes laminin, also known as LAMB1 or laminin subunit beta-1.

      Laminin subunits form the heterotrimeric extracellular matrix protein that is essential for basement membrane assembly and embryonic development.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/10001/4000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti laminin antibody detects a band of approximately 198 kDa in cell lysates and tissue lysates under reducing conditions
    • Instructions For Use

    Additional Laminin Antibody Formats

    Formats Applications Sizes available
    Laminin Antibody : Purified WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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