Laminin Antibody | 5H2

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  • Mouse anti Human Laminin Alpha 2
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  • Product Type
    Monoclonal Antibody
  • Clone
    5H2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6067-0906C *, E, IF, IP, WBdatasheet pdfdatasheet pdf50 µl
    6067-0906
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • 6067-0906 reacts with the 80 kD fragment of the M-chain of human Laminin alpha2, originally known as Merosin. Laminins are trimeric basement glycoproteins consisting of three non-identical multi-domain chains (alpha, beta and gamma) each encoded by a distinct gene. The alpha2 chain is a subunit of Laminin-2 and Laminin-4, which are widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminins are thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rabbityes
      Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ascitic Fluid - raw
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Purified Human Merosin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      structural molecule activity
      regulation of cell migration
      regulation of embryonic development
      laminin-1 complex
      regulation of cell adhesion
      receptor binding
      cell adhesion
      muscle organ development
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/250001/100000
      Immunofluorescence
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)1/20001/10000
      (1)
      Acetone is recommended. 8 µm fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Laminin Antibody Formats

    Formats Clone Applications Sizes available
    Laminin Antibody : Ascites 5H2 C *, E, IF, IP, WB 50 µl
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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