Laminin Antibody | 5H2

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Laminin Antibody | 5H2 gallery image 1

Published investigator image:
Mouse anti Human Laminin Alpha 2 antibody, clone 5H2 used for the immunopurification of laminin alpha-2 from human placenta for coating culture vessels prior to plating of airway smooth muscle cells.
Image caption:
Phase contrast images of ASM cells in the presence and absence of different coating of laminin isoforms following 7-day serum deprivation. EHS = laminin from Engelbreth-Holm-Swarm murine sarcoma; β1 = affinity purified β1 chain-containing laminin from human placenta that includes LN-1, 2, 6, 8, 10; α2 = affinity purified α2 chain-containing laminin from human placenta that includes LN-2 and 4. Bar = 70 μm.

From: Tran T, McNeill KD, Gerthoffer WT, Unruh H, Halayko AJ.
Endogenous laminin is required for human airway smooth muscle cell maturation.
Respir Res. 2006 Sep 12;7:117.

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Laminin Antibody | 5H2 gallery image 2

Published investigator image:
Mouse anti Human Laminin Alpha 2 antibody, clone 5H2 used for the evaluation of laminin alpha-2 expression in cultured human airway smooth muscle cells by western blotting.
Image caption:
Western blot analysis showing the protein abundance of laminin chains at Day 0 (open bars) and following 7-day serum deprivation (closed bars). † P<0.05, compared with the respective laminin chain at Day 0.

From: Tran T, McNeill KD, Gerthoffer WT, Unruh H, Halayko AJ.
Endogenous laminin is required for human airway smooth muscle cell maturation.
Respir Res. 2006 Sep 12;7:117.

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  • Mouse anti Human Laminin Alpha 2
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  • Product Type
    Monoclonal Antibody
  • Clone
    5H2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6067-0906C *, E, IF, IP, WBdatasheet pdfdatasheet pdf50 µl
    6067-0906
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human laminin alpha 2 antibody, clone 5H2 recognizes the ~80 kDa fragment of laminin subunit alpha-2, also known as the laminin M chain, laminin-12 subunit alpha, laminin-2 subunit alpha, laminin-4 subunit alpha or merosin heavy chain. Laminins are trimeric basement glycoproteins consisting of three non-identical multi-domain chains (alpha, beta and gamma) each encoded by a distinct gene. The alpha-2 chain is a subunit of Laminin-2 and Laminin-4, widely expressed in the basement membrane of skeletal muscle and peripheral nerves. Laminins are thought to mediate cell attachment, migration, proliferation and differentiation with other extracellular matrix components.

      Mutation of the LAMA2 gene can lead to the development of Merosin-deficient congenital muscular dystrophy 1A (MDC1A), a condition characterized by hypotonia, proximal weakness, hyporeflexia and difficulty walking (Tazek et al. 2003).
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rabbityes
      Monkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ascitic Fluid - raw
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Purified Human Merosin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      structural molecule activity
      regulation of cell migration
      regulation of embryonic development
      laminin-1 complex
      regulation of cell adhesion
      receptor binding
      cell adhesion
      muscle organ development
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/250001/100000
      Immunofluorescence
      Immunoprecipitation
      Western Blotting
      Immunohistology - Frozen(1)1/20001/10000
      (1)
      Acetone is recommended. 8 µm fixed cryostat muscle sections have been used, with an HRP conjugated secondary antibody for detection.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Laminin Antibody Formats

    Formats Clone Applications Sizes available
    Laminin Antibody : Ascites 5H2 C *, E, IF, IP, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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