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Mouse anti Human Laminin Alpha 2 antibody, clone 5H2 used for the immunopurification of laminin alpha-2 from human placenta for coating culture vessels prior to plating of airway smooth muscle cells.
Phase contrast images of ASM cells in the presence and absence of different coating of laminin isoforms following 7-day serum deprivation. EHS = laminin from Engelbreth-Holm-Swarm murine sarcoma; β1 = affinity purified β1 chain-containing laminin from human placenta that includes LN-1, 2, 6, 8, 10; α2 = affinity purified α2 chain-containing laminin from human placenta that includes LN-2 and 4. Bar = 70 μm.
From: Tran T, McNeill KD, Gerthoffer WT, Unruh H, Halayko AJ.
Endogenous laminin is required for human airway smooth muscle cell maturation.
Respir Res. 2006 Sep 12;7:117.