Ku70 Antibody (PrecisionAbTM Antibody)

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Ku70 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with Ku70 antibody followed by detection with HRP conjugated Goat anti Human IgG F(ab')2 (1/5000, STAR126P) and visualized on the ChemiDoc MP with 20 second exposure. Arrow points to Ku70 (molecular weight 70 kDa)

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  • Human anti Ku70
  • Ku70 Antibody with Control Lysate
  • Ku70 Antibody with Control Lysate
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    Ku70 antibody recognizes X-ray repair cross-complementing protein 6, also known as CTC75, CTCBF, DNA repair protein XRCC6, or thyroid-lupus autoantigen. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      HuCAL Fab bivalent
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00013WBdatasheet pdfdatasheet pdf100 µl
      VMA00013
      VMA00013KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00013K
      VMA00013KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00013KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Human anti Human Ku70 antibody recognizes Human Ku70, also known as X-ray repair cross-complementing protein 6 or XRCC6. Ku70 is a 609 amino acid, nuclear protein involved in DNA damage repair. Ku70 contains a single Ku domain and a single SAP domain.

        Ku70 forms a hetrodimer comprising of p70/p86 which acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA. Research indicates that the Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together (Walker et al. 2001).

        Human anti Human Ku70 antibody recognizes human Ku70 as a single band of ~70 kDa in multiple human cell line lysates by western blotting under reducing conditions.
      • Target Species
        Human
      • Western Blotting
        Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
        Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
        Anti Ku70 antibody detects a band of approximately 70 kDa in HeLa cell lysates.
      • Product Form
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
        A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a double extended Strep-tag at the C-terminus of the antibody heavy chain. This product is supplied as a liquid.
      • Preparation
        Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 10 Westerns100μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
        Pack Size: 2 Westerns20μl Recombinant human monoclonal antibody purified by metal chelate affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Human Ku70 - N1 fusion protein
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HeLa lysate lyophilized in RIPA buffer.
        400μg HeLa lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        membrane fraction
        Ku70:Ku80 complex
        5'-deoxyribose-5-phosphate lyase activity
        nuclear telomere cap complex
        double-strand break repair via nonhomologous end joining
        DNA ligation
        telomere maintenance
        transcription
        negative regulation of transcription
        DNA-dependent protein kinase-DNA ligase 4 complex
        ATP-dependent DNA helicase activity
        transcription factor complex
        positive regulation of gene-specific transcription from RNA polymerase II promoter
        promoter binding
        provirus integration
        protein C-terminus binding
        initiation of viral infection
        ATP binding
        double-stranded DNA binding
        transcription activator activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        MCA2489P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        MCA2489P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
        STAR126P
        Mouse anti Strep-Tag Classic:HRPMCA2489P75 µlE, WB
        MCA2489P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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