Ku70 Antibody | 4C2-1A6

Ku70 Antibody | 4C2-1A6 gallery image 1

Western blot analysis of Ku70 expression in C32 human amelanotic melanoma lysate using Mouse anti Human Ku70:Azide Free (MCA3127Z)

Ku70 Antibody | 4C2-1A6 gallery image 2

Immunofluorescence showing Ku70 in HeLa cells using Mouse anti Human Ku70:Azide Free (MCA3127Z)

Ku70 Antibody | 4C2-1A6 gallery image 3

Staining of paraffin embedded human breast cancer with Mouse anti Human Ku70:Azide Free (MCA3127Z)

Ku70 Antibody | 4C2-1A6 gallery image 4

Western blot analysis of MDA-MB-231 human breast adenocarcinoma whole cell lysate probed with Mouse anti Human Ku70 antibody (MCA3127Z) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence

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  • Mouse anti Human Ku70:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA3127ZE, IF, P*, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human Ku70 antibody, clone 4C2-1A6 recognizes human X-ray repair cross-complementing protein 6 (XRCC6), also known as Ku70, Lupus Ku autoantigen protein p70, CTC box-binding factor 75 kDa subunit or ATP-dependent DNA helicase 2 subunit 1. Ku70 is a 609 amino acid ~70 kDa ATP dependent helicase involved in chromosome translocation (UniProt: P12956).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA15 ug/ml
      Immunofluorescence520 ug/ml
      Western Blotting15 ug/ml
      Immunohistology - Paraffin(1)15 ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human breast cancer.
    • Immunofluorescence
    • Western Blotting
      Mouse anti Human Ku70 antibody, clone 4C2-1A6 detects a band of ~70 kDa in western blotting using lysates from either C32 cell lines or XRCC6-transfected 293T cell lines.
    • Instructions For Use

    Additional Ku70 Antibody Formats

    Formats Clone Applications Sizes available
    Ku70 Antibody : Preservative Free 4C2-1A6 E, IF, P*, WB 0.1 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human breast cancer.


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