Krueppel-Like Factor 4 Antibody (PrecisionAbTM Antibody)

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Krueppel-Like Factor 4 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with Krueppel-like factor 4 antibody followed by detection with Goat anti Rabbit HRP conjugated(1/10,000,STAR208P) and visualized on the ChemiDoc MP with 63 second exposure. Arrow points to Krueppel-like factor 4 (molecular weight 62 kDa)

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  • Krueppel-Like Factor 4 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Krueppel-Like Factor 4 Antibody with Control Lysate
  • Rabbit anti Krueppel-Like Factor 4
  • Krueppel-Like Factor 4 Antibody with Control Lysate
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    Krüeppel-like factor 4 antibody recognizes Krüeppel-like factor 4, also known as KIF4, endothelial Krüppel-like zinc finger protein, epithelial zinc finger protein EZF or gut-enriched Krüeppel-like factor. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00223WBdatasheet pdfdatasheet pdf100 µl
      VPA00223
      VPA00223KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00223K
      VPA00223KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00223KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human Krueppel-like factor 4 antibody recognizes Krueppel-like factor 4, also known as endothelial Kruppel-like zinc finger protein, epithelial zinc finger protein EZF and gut-enriched krueppel-like factor.

        Rabbit anti Human Krueppel-like factor 4 antibody detects a band of 62 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti Krueppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
        Anti Kreuppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
        Anti Krueppel-like factor 4 detects a band of approximately 62 kDa in HepG2 cell lysates
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 10 Westerns100μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
        Pack Size: 2 Westerns20μl Rabbit Ig fraction prepared by ammonium sulphate precipitation
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH conjugated synthetic peptide between 20-53 amino acids from the N-terminal region of human Krueppel-like factor 4
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HepG2 lysate lyophilized in RIPA buffer.
        400μg HepG2 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        gene-specific transcription from RNA polymerase II promoter
        negative regulation of cell proliferation
        transcription activator activity
        transcription repressor activity
        RNA polymerase II transcription factor binding
        nucleus
        mesodermal cell fate determination
        zinc ion binding
        negative regulation of transcription, DNA-dependent
        promoter binding
        stem cell maintenance
        fat cell differentiation
        sequence-specific DNA binding transcription factor recruiting transcription factor activity
        positive regulation of gene-specific transcription from RNA polymerase II promoter
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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