KPNA1 Antibody

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KPNA1 Antibody gallery image 1

Western blot analysis of cell and tissue lysates using Rabbit anti KPNA1 antibody

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KPNA1 Antibody gallery image 2

Immunohistochemistry of paraffin-embedded human lung cancer using Rabbit anti KPNA1 antibody at a dilution of 1/100

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KPNA1 Antibody gallery image 3

Immunohistochemistry of paraffin-embedded human esophageal tissue using Rabbit anti KPNA1 antibody at a dilution of 1/100

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  • Rabbit anti KPNA1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2489P, WBdatasheet pdfdatasheet pdf50 µl
    AHP2489
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti KPNA1 antibody recognizes KPNA1, also known as karyopherin alpha 1, importin subunit alpha-5, importin 5 and RCH2.

      KPNA1 belongs to the importin alpha family and is involved in the import of nuclear proteins. Nuclear trafficking mediated by KPNA1 plays a key role in regulating development and differentiation.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      RatExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to KPNA1 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide
      50% Glycerol
    • Immunogen
      Recombinant human KPNA1
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      regulation of DNA recombination
      viral infectious cycle
      cytosol
      nucleoplasm
      protein transporter activity
      DNA fragmentation involved in apoptotic nuclear change
      nuclear localization sequence binding
      nuclear pore
      protein binding
      viral genome transport in host cell
      NLS-bearing substrate import into nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin1/501/200
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti KPNA1 antibody detects a band of approximately 60 kDa in cell lysates and tissue lysates under reducing conditions
    • Instructions For Use

    Additional KPNA1 Antibody Formats

    Formats Applications Sizes available
    KPNA1 Antibody : Purified P, WB 50 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
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      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
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      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

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