Ki67 Antibody | AbD02815
Paraffin embedded human tonsil stained with Human anti Human Ki67 (HCA117) after citrate antigen retrieval
Enlarge- Human anti Ki67
- Human anti Ki67
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Product TypeMonoclonal Antibody
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CloneAbD02815
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IsotypeHuCAL Fab bivalent
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Human anti Ki67 antibody, clone AbD02815 recognizes the sequence GFKELFQ, which can be found eight times on the human Ki67 protein. Ki67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki67 is expressed in the nucleolus of interphase cells.
The Ki67 gene contains 15 exons. The Ki67 repeat region, within which there is a 22-amino acid Ki67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis revealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis showed expression of 320 and 359 kDa proteins. Sequence analysis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular proliferation in a dose-dependent manner, suggesting that Ki67 protein expression may be an absolute requirement for cell proliferation.
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Intended Use
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Target SpeciesHuman
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Product FormPack Size: 0.1 mgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.Pack Size: 50 µgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.Pack Size: 0.1 mgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.Pack Size: 50 µgA bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
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ReconstitutionPack Size: 50 µgReconstitute with 0.5 ml distilled waterPack Size: 50 µgReconstitute with 0.5 ml distilled water
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Preparationmetal chelate affinity chromatographymetal chelate affinity chromatography
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Preservative StabilisersPack Size: 0.1 mg0.01% ThiomersalPack Size: 50 µg0.09% Sodium Azide (NaN3)
1% Bovine Serum AlbuminPack Size: 0.1 mg0.01% ThiomersalPack Size: 50 µg0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin -
ImmunogenPeptide derived from the human Ki67 protein, sequence GFKELFQTPG, coupled via a C-terminal cysteine to carrier proteins
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Purity
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Approx. Protein ConcentrationsPack Size: 0.1 mgTotal protein concentration 0.5 mg/mlPack Size: 50 µgTotal protein concentration 0.1 mg/ml after reconstitutionPack Size: 0.1 mgTotal protein concentration 0.5 mg/mlPack Size: 50 µgTotal protein concentration 0.1 mg/ml after reconstitution
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered salinePhosphate buffered saline
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StoragePack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µgPrior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 50 µgPrior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. -
Shelf Life12 months from date of despatch12 months from date of reconstitution
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GO TermsnucleolusATP bindingprotein C-terminus bindingcell proliferation
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UniProt
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Entrez Gene
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AcknowledgementsSold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences. -
Licensed UseFor in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Immunohistology - Paraffin(1) 1/50 1/200 (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.Application Name Yes No Min Dilution Max Dilution ELISA Immunohistology - Paraffin(1) 1/50 1/200 (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical AdviceRecommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Technical AdviceRecommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Recommended Protocol
- Recommended Protocol
- ELISA
- ELISAThis product is suitable for use in direct ELISA applications. Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
- Immunohistology
- Immunohistology
- Histology Positive Control TissueHuman tonsil
- Histology Positive Control TissueHuman tonsil
- Immunofluorescence
- Immunofluorescence
- Western Blotting
- Western Blotting
- Instructions For Use
- Instructions For Use
Additional Ki67 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| Ki67 Antibody : Purified | AbD02815 | E, P* | 0.1 mg | 50 µg |
- Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat anti DYKDDDDK Tag:HRP | MCA4764P | 0.1 mg | E, P, WB | MCA4764P | |
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E, WB | MCA5995P |
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat anti DYKDDDDK Tag:HRP | MCA4764P | 0.1 mg | E, P, WB | MCA4764P | |
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | 125 µl | E, WB | MCA5995P |
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
Human tonsil
Human tonsil
