Ki67 Antibody | 2531

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Paraffin embedded Human Tonsil stained with Human anti Ki-67 antibody, clone 2531 (HCA053)

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  • Human anti Ki67
  • Human anti Ki67
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2531
  • Isotype
    HuCAL/mouse IgG1 Fc
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA053C, E, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA053
    HCA053AC, E, P *, WBdatasheet pdfdatasheet pdf50 µg
    HCA053A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Human anti Ki-67 antibody, clone 2531 recognizes the Ki-67 cell-cycle assocaited protein. Ki-67 is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis reveals multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis shows expression of 320 and 359 kDa proteins. Antisense oligonucleotides inhibit cellular proliferation in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.

      Within cells Ki67 is predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      BovineExpected from Sequence
      MacaqueExpected from Sequence
      DogExpected from Sequence
      ChimpanzeeExpected from Sequence
      Rhesus MonkeyExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Pack Size: 0.1 mgChimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied liquid.
      Pack Size: 50 µgChimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied lyophilised.
      Pack Size: 0.1 mgChimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied liquid.
      Pack Size: 50 µgChimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 50 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein A
      Pack Size: 50 µgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Peptide derived from the human Ki-67 protein. Sequence GFKELFQTPG, coupled via a C-terminal cysteine to carrier proteins.
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 50 µgIgG concentration 0.1mg/ml
      Pack Size: 0.1 mgIgG concentration 0.5mg/ml
      Pack Size: 50 µgIgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA2ug/ml
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      0.2ug/mlPack Size: 0.1 mg
      1/100Pack Size: 50 µg
      1.0ug/mlPack Size: 0.1 mg
      1/500Pack Size: 50 µg
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA2ug/ml
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      0.2ug/mlPack Size: 0.1 mg
      1/100Pack Size: 50 µg
      1.0ug/mlPack Size: 0.1 mg
      1/500Pack Size: 50 µg
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA, please enquire.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Human tonsil
    • Histology Positive Control Tissue
      Human tonsil
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Ki67 Antibody Formats

    Formats Clone Applications Sizes available
    Ki67 Antibody : Purified 2531 C, E, P *, WB 50 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      0500-0099
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      STAR3000A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Histar Detection SystemSTAR3000A50 TestsC, P
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Human tonsil
              Human tonsil

              References

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