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KGF Antibody

KGF Antibody gallery image 1

Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (AHP783) following antigen retrieval using proteinase K

KGF Antibody gallery image 2

Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (AHP783) following antigen retrieval using proteinase K

  • KGF Antibody thumbnail image 1
  • KGF Antibody thumbnail image 2
  • Rabbit anti Human KGF
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP783E, FN, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin.

      KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
    • Preparation
    • Antiserum Preparation
      Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human KGF.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      positive regulation of peptidyl-tyrosine phosphorylation
      chemoattractant activity
      positive regulation of keratinocyte proliferation
      positive regulation of keratinocyte migration
      positive regulation of cell division
      actin cytoskeleton reorganization
      insulin receptor signaling pathway
      growth factor activity
      mesenchymal cell proliferation
      fibroblast growth factor receptor signaling pathway
      secretion by lung epithelial cell involved in lung growth
      protein localization at cell surface
      positive regulation of epithelial cell proliferation involved in lung morphogenesis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays0.560.85ug/ml
      Western Blotting0.10.2ug/ml
      Immunohistology - Paraffin(1)
      This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional KGF Antibody Formats

    Formats Applications Sizes available
    KGF Antibody : Purified E, FN, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • KGF Antibody thumbnail image 1
                • KGF Antibody thumbnail image 2

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