Western blot analysis of mouse brain tissue extract probed with HuCal anti JNK3 (AbyD02547) followed by mouse anti histidine tag:HRP (MCA5995P). Note the presence of two bands, consistent with the reported presence of two isoforms of JNK3
HCA030 recognises the human protein tyrosine kinase JNK3, a protein of approximately 45kD.
JNK3 acts to phosphorylate a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity in response to stress. JNK3 is required for stress-induced neuronal apoptosis.
JNK3 is expressed predominantly in brain, with very weak expression reported in testis and kidney. Two isoforms of JNK3 are recognised due to alternative gene splicing.
N.B. Antibody reactivity and working conditions may vary between species.
A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
Reconstitute with 1.0ml distilled water
Metal chelate affinity chromatography
0.09% Sodium Azide (NaN3)
Recombinant human JNK3
Approx. Protein Concentrations
Antibody concentration 0.1mg/ml following reconstitution
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.