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JNK3 Antibody | 2547

JNK3 Antibody | 2547 gallery image 1

HCA030 binding in ELISA to JNK3 protein, demonstrating no cross-reactivity with other tested proteins

JNK3 Antibody | 2547 gallery image 2

Western blot analysis of mouse brain tissue extract probed with HuCal anti JNK3 (AbyD02547) followed by mouse anti histidine tag:HRP (MCA5995P).
Note the presence of two bands, consistent with the reported presence of two isoforms of JNK3

  • JNK3 Antibody | 2547 thumbnail image 1
  • JNK3 Antibody | 2547 thumbnail image 2
  • Human anti JNK3
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA030E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • HCA030 recognises the human protein tyrosine kinase JNK3, a protein of approximately 45kD.

      JNK3 acts to phosphorylate a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity in response to stress. JNK3 is required for stress-induced neuronal apoptosis.

      JNK3 is expressed predominantly in brain, with very weak expression reported in testis and kidney.
      Two isoforms of JNK3 are recognised due to alternative gene splicing.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
    • Reconstitution
      Reconstitute with 1.0ml distilled water
    • Preparation
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant human JNK3
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 0.1mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      6 months from date of reconstitution.
    • GO Terms
      toll-like receptor 3 signaling pathway
      MyD88-dependent toll-like receptor signaling pathway
      regulation of transcription factor activity
      toll-like receptor 2 signaling pathway
      toll-like receptor 1 signaling pathway
      MyD88-independent toll-like receptor signaling pathway
      protein binding
      Toll signaling pathway
      stress-activated MAPK cascade
      ATP binding
      JUN kinase activity
      MAP kinase kinase activity
      toll-like receptor 4 signaling pathway
      innate immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional JNK3 Antibody Formats

    Formats Clone Applications Sizes available
    JNK3 Antibody : Purified 2547 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • JNK3 Antibody | 2547 thumbnail image 1
                • JNK3 Antibody | 2547 thumbnail image 2

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