Jnk (pThr183/pTyr185) Antibody | D04-7G6

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Jnk (pThr183/pTyr185) Antibody | D04-7G6 gallery image 1

Western blot analysis of total (left) and T183/Y185 phosphorylated (right) JNK. HEK293 cells were either untreated, treated with phosphatase or exposed to UV radiation. T183/Y185 phosphorylated JNK levels were analysed using Mouse anti Human JNK (p Thr183/p Tyr 185) (MCA5991GA)

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  • Mouse anti Jnk (pThr183/pTyr185)
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  • Product Type
    Monoclonal Antibody
  • Clone
    D04-7G6
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5991GAE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5991GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human JNK (p Thr183/p Tyr185) antibody, clone D04-7G6 recognizes the c-jun N-terminal kinase (JNK) when phosphorylated at residues T183/Y185. Phosphorylation of these residues is associated with the activation of JNK following exposure to enviromental stress (e.g. UV).

      JNKs are members of the mitogen activated protein kinase (MAPK) family which constitute several signalling pathways. In this signalling cascade extracellular stimuli activate small GTAPases (of the Rho family) which in turn acitvate MAP2K4/MKK4 and MAP2K7/MKK7. These kinases then in turn activate JNK. JNK then interacts with a variety of downstream targets including other signalling molcules and transcription factors (Sehgal & Ram, 2013).
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from ascites
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to residues surrounding T183/Y185 of human JNK
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BLAB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Jnk (pThr183/pTyr185) Antibody Formats

    Formats Clone Applications Sizes available
    Jnk (pThr183/pTyr185) Antibody : Purified D04-7G6 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              Further Reading

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