JARID1B antibody | 1G10
Mouse anti Human JARID1B antibody, clone 1G10 (MCA4340Z) used to evaluate expression of the lower molecular weight form of KDM5B in MCF7 cell lysates by western blotting.
Image caption:
WB analysis of KDM5B isoform expression in MCF7 lysates: on the left two bands corresponding to 175.7 and 161.5 kDa proteins are detected by Ab1 in the first blot, while on the right one single band for 161.5 kDa protein is detected by Ab2, after the stripping of the first blotting. D Preliminary investigation of KDM5B isoforms’ expression in different human cell lines and human testis, using the two antibodies Ab1 (panel up) and Ab2 (panel down), before and after the stripping, respectively.
From: Di Nisio E, Licursi V, Mannironi C, Buglioni V, Paiardini A, Robusti G, Noberini R, Bonaldi T, Negri R.
A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression.
Cancer Gene Ther. 2023 Jan 26 [Epub ahead of print].
doi: 10.1038/s41417-022-00584-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human JARID1B antibody, clone 1G10 (MCA4340Z) used to evaluate expression of the lower molecular weight form of KDM5B in human cell lysates by western blotting.
Image caption:
After CHX treatment, the KDM5B-NTT results more stable than KDM5B-PLU-1 in both MCF7 and MDA-MB-231, as shown from the blots using the anti-C-terminal KDM5B (upper panel) and the anti-exon-6 KDM5B (lower panel) antibodies.
From: Di Nisio E, Licursi V, Mannironi C, Buglioni V, Paiardini A, Robusti G, Noberini R, Bonaldi T, Negri R.
A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression.
Cancer Gene Ther. 2023 Jan 26 [Epub ahead of print].
doi: 10.1038/s41417-022-00584-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human JARID1B antibody, clone 1G10 (MCA4340Z) used to evaluate expression of the lower molecular weight form of KDM5B in human cell lysates by western blotting.
Image caption:
WB analysis of KDM5B isoforms’ expression in lysates of different breast cancer cell lines shows that NTT isoform is detected in all of them; as confirmed also by the blotting using the Ab2, the exon-6-encoded residues are almost exclusively included in the truncated isoform. Cell lines are grouped according to the molecular subtypes.
From: Di Nisio E, Licursi V, Mannironi C, Buglioni V, Paiardini A, Robusti G, Noberini R, Bonaldi T, Negri R.
A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression.
Cancer Gene Ther. 2023 Jan 26 [Epub ahead of print].
doi: 10.1038/s41417-022-00584-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human JARID1B:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 1G10
- Isotype
- IgG2a
- Specificity
- JARID1B
Antibody Quality and Performance Guarantee
| Mouse anti Human JARID1B antibody, clone 1G10 recognizes human Lysine-specific demethylase 5B, also known as JARID1B, Cancer/testis antigen 31, Histone demethylase JARID1B, PLU-1, Retinoblastoma-binding protein 2 homolog 1 or Jumonji/ARID domain-containing protein 1B. JARID1B is a 1544 amino acid ~180 kDa ubiquitously expressed histone demethylase. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 231-320 of human JARID1B
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
0.1 | 10 ug/ml |
| Western Blotting | |
0.1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for JARID1B antibody
-
Kuźbicki, L. et al. (2013) JARID1B expression in human melanoma and benign melanocytic skin lesions.
Melanoma Res. 23 (1): 8-12. -
Kużbicki, Ł. et al. (2017) Prognostic significance of RBP2-H1 variant of JARID1B in melanoma.
BMC Cancer. 17 (1): 854. -
Di Nisio, E. et al. (2023) A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression.
Cancer Gene Ther. Jan 26 [Epub ahead of print].
Further Reading
-
Xiang, Y. et al. (2007) JARID1B is a histone H3 lysine 4 demethylase up-regulated in prostate cancer.
Proc Natl Acad Sci U S A. 104: 19226-31.
- Synonyms
- Lysine Specific Demethylase 5B
- RRID
- AB_1221667
- UniProt
- Q9UGL1
- Entrez Gene
- KDM5B
- GO Terms
- GO:0005515 protein binding
- GO:0003677 DNA binding
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0005730 nucleolus
- GO:0055114 oxidation-reduction process
- GO:0008270 zinc ion binding
- GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
- GO:0034647 histone demethylase activity (H3-trimethyl-K4 specific)
- View More GO Terms
- GO:0034648 histone demethylase activity (H3-dimethyl-K4 specific)
- GO:0045892 negative regulation of transcription, DNA-dependent
MCA4340Z
158024
170320
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