IRGM antibody
Rabbit anti IRGM (Internal Region)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IRGM
- Region
- (INTERNAL REGION)
Antibody Quality and Performance Guarantee
| Rabbit anti IRGM (Internal Region) antibody recognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47. IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy. IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to a 14 aa sequence from near the centre of human IRGM.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
20ug/ml | |
| Immunohistology - Paraffin 1 | |
5ug/ml | |
| Western Blotting | |
1 | 2ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human brain
- Western Blotting
- AHP1887 detects a band of approximately 32 kDa in human brain cell lysate.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Antigen Retrieval Buffer, pH8.0 | ||||||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB * | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
- RRID
- AB_1898227
- UniProt
- A1A4Y4
- Q6AYC2
- Q60766
- Entrez Gene
- IRGM
- Irgm1
- Irgm
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0000421 autophagic vacuole membrane
- GO:0005525 GTP binding
- GO:0006914 autophagy
- GO:0006954 inflammatory response
- GO:0016817 hydrolase activity, acting on acid anhydrides
- GO:0030670 phagocytic vesicle membrane
- GO:0042995 cell projection
- View More GO Terms
- GO:0045087 innate immune response
- GO:0005783 endoplasmic reticulum
- GO:0005765 lysosomal membrane
- GO:0031902 late endosome membrane
AHP1887
152880
161174
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