IRGM Antibody

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IRGM Antibody gallery image 1

Western blot analysis of rat brain lysate probed with Rabbit anti Human IRGM (AHP1886) at 1(A) and 2(B) ug/ml

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  • IRGM Antibody thumbnail image 1
  • Rabbit anti IRGM (N-Terminal)
  • Rabbit anti IRGM (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1886WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1886
    AHP1886TWBdatasheet pdfdatasheet pdf50 µg
    AHP1886T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti IRGM (N-Terminal) antibody recognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47.

      IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy.

      IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.02% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to a 17 aa sequence from near the amino terminus of human IRGM.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      innate immune response
      lysosomal membrane
      inflammatory response
      hydrolase activity, acting on acid anhydrides
      plasma membrane
      cell projection
      Golgi membrane
      phagocytic vesicle membrane
      autophagic vacuole membrane
      endoplasmic reticulum
      late endosome membrane
      autophagy
      GTP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting12ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting12ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      AHP1886 detects a band of approximately 32 kDa in Rat brain cell lysate.
    • Western Blotting
      AHP1886T detects a band of approximately 32 kDa in Rat brain cell lysate.
    • Instructions For Use
    • Instructions For Use

    Additional IRGM Antibody Formats

    Formats Applications Sizes available
    IRGM Antibody : Purified WB 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A

          Recommended Positive Controls

            Histology Controls

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