IRF3 Antibody | 3F10

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IRF3 Antibody | 3F10 gallery image 1

Western blot analysis of IRF3 expression on a Jurkat cell lysate using Mouse anti Human IRF3 (MCA2700)

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IRF3 Antibody | 3F10 gallery image 2

Imminoprecipitaion of IRF3 fron HeLa cells using Mouse anti Human IRF3 (MCA2700)
Lane A: Normal IgG
Lane B: Mouse anti Human IRF3, clone 3F10
(non-specific bands represent IgG H+L in the preparation)

Immunofluorescent staining of HeLa cells with Mouse anti Human IRF3 (MCA2700), IRF3 (Red), nuclei counterstained with Hoescht 3342 (blue)

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  • IRF3 Antibody | 3F10 thumbnail image 1
  • IRF3 Antibody | 3F10 thumbnail image 2
  • Mouse anti Human IRF3
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    3F10
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2700E, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2700
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Human IRF3 antibody, clone 3F10 recognizes human interferon regulatory factor 3 (IRF3), a 55kDa constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity (Nociari et al. 2007).

      IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta and subsequent downstream interferon-induced genes (Weaver et al. 1998).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting1/10001/3000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2700 detects a band of approximately 55kDa in HeLa cell lysates.
    • Instructions For Use

    Additional IRF3 Antibody Formats

    Formats Clone Applications Sizes available
    IRF3 Antibody : Purified 3F10 E, IP, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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          Recommended Positive Controls

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